Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads. Effects of starvation, alloxan-diabetes and high-fat diet.

The Biochemical journal ·Vol. 154 ·No. 1 ·1976-01-15 ·Pages 225-36

Stansbie D, Denton RM, Bridges BJ, Pask HT, Randle PJ

Abstract

1. Pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads was measured by using pig heart pyruvate dehydrogenase [32P]phosphate. About 80% was found to be extramitochondrial and therefore probably not directly concerned with the regulation of pyruvate dehydrogenase activity. The extramitochondrial activity was sensitive to activation by Ca2+, but perhaps less sensitive than the mitochondrial activity.

MeSH Terms

Adipose Tissue/drug effects,enzymology Animals Calcium/pharmacology Diabetes Mellitus, Experimental/metabolism Dietary Fats Epididymis/enzymology Glucose/metabolism In Vitro Techniques Insulin/pharmacology Malate Dehydrogenase/metabolism Male NADP Phosphoric Monoester Hydrolases/metabolism Pyruvate Dehydrogenase (Lipoamide)-Phosphatase/metabolism Pyruvate Dehydrogenase Complex/metabolism Rats Starvation Time Factors

Chemicals

Dietary Fats Insulin Pyruvate Dehydrogenase Complex NADP Malate Dehydrogenase Phosphoric Monoester Hydrolases Pyruvate Dehydrogenase (Lipoamide)-Phosphatase Glucose Calcium

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Stansbie D

Denton R M

Bridges B J

Pask H T

Randle P J

References (35)

35 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1540225

Published

1976-01-15

Pages

225-36

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1172695

Subset

External Links

PubMed source DOI: 10.1042/bj1540225 PMC Full Text View journal details

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Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads. Effects of starvation, alloxan-diabetes and high-fat diet.

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