Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes.

Chromosoma ·Vol. 57 ·No. 2 ·1976-08-17 ·Pages 165-9

Howell WM, Denton TE

Abstract

Human metaphase chromosomes were stained with silver following a pretreatment with a heated alkaline solution. The most conspicuous feature of the stained metaphases was the omission of silver staining in the secondary constrictions of chromosomes 1,9 and 16, and on the distal Yq. Our evidence indicates that the negative silver binding is due to the preferential removal or alteration of non-histone proteins associated with these regions. The cytochemical significance of these findings is discussed.

MeSH Terms

Adenine Nucleotides/analysis Chromosomes/analysis Chromosomes, Human, 1-3 Chromosomes, Human, 16-18 Chromosomes, Human, 6-12 and X DNA/analysis DNA, Satellite/analysis Humans Sex Chromosomes/analysis Silver Staining and Labeling Thymine Nucleotides/analysis

Chemicals

Adenine Nucleotides DNA, Satellite Thymine Nucleotides Silver DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Howell W M

Denton T E

References (9)

9 references, click to expand

Differential staining of the satellite regions of human acrocentric chromosomes.
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The chromosomal localisation of human satellite DNA I.
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Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl.
Stain Technol. 1965 Nov;40(6):333-8 PMID: 5866557
Acridine-orange differential fluorescence of fast- and slow-reassociating chromosomal DNA after in situ DNA denaturation and reassociation.
Chromosoma. 1972;37(2):117-30 PMID: 4114487
An ammoniacal-silver stain technique specific for satellite III DNA regions on human chromosomes.
Experientia. 1974 Nov 15;30(11):1364-6 PMID: 4140108
Chromosome regions containing DNAs of known base composition, specifically evidenced by 2,7-di-t-butyl proflavine. Comparison with the Q-banding and relation to dye-DNA interactions.
Chromosoma. 1974;47(3):263-81 PMID: 4139005
Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.
Chromosoma. 1975 Nov 20;53(1):37-50 PMID: 53131
The relationship between patterns of DNA replication and of quinacrine fluorescence in the human chromosome complement.
Chromosoma. 1971;35(3):326-41 PMID: 4109087
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Exp Cell Res. 1973 Mar 15;77(1):469-83 PMID: 4120442

Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00292914

Published

1976-08-17

Pages

165-9

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00292914 View journal details

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Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes.

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