Artifact produced in disc electrophoresis by ammonium persulfate.

Science (New York, N.Y.) ·Vol. 156 ·No. 3772 ·1967-04-14 ·Pages 256-7

Brewer JM

Abstract

Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.

MeSH Terms

Acrylic Resins Electrophoresis Glycolates Hydro-Lyases/analysis Light Quaternary Ammonium Compounds Riboflavin Sulfates Thioglycolates Yeasts/enzymology

Chemicals

Acrylic Resins Glycolates Quaternary Ammonium Compounds Sulfates Thioglycolates Hydro-Lyases Riboflavin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Brewer J M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.156.3772.256

Published

1967-04-14

Pages

256-7

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.156.3772.256 View journal details

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Artifact produced in disc electrophoresis by ammonium persulfate.

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