The action of cabbage-leaf phospholipase D upon lysolecithin.

The Biochemical journal ·Vol. 102 ·No. 1 ·1967-01-00 ·Pages 216-20

Long C, Odavić R, Sargent EJ

Abstract

1. The action of the water-soluble phospholipase D from Savoy cabbage leaves upon an aqueous solution of lysolecithin has been studied. 2. Optimum conditions required the presence of Ca(2+) ions and pH5.8. 3. Equivalence was found between the amounts of lysolecithin hydrolysed and free choline released. The reaction was not accompanied by deacylation. 4. As the enzymic degradation proceeded, an opalescence developed, owing to the insolubility of the reaction product in the presence of Ca(2+) ions. 5. Evidence has been obtained indicating the heterogeneity of this reaction product.

MeSH Terms

Calcium Chemical Phenomena Chemistry Chromatography, Thin Layer Hydrogen-Ion Concentration In Vitro Techniques Lysophosphatidylcholines/metabolism Phospholipases/metabolism Plants, Edible/enzymology

Chemicals

Lysophosphatidylcholines Phospholipases Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Long C

Odavić R

Sargent E J

References (14)

14 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1020216

Published

1967-01-00

Pages

216-20

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270231

Subset

External Links

PubMed source DOI: 10.1042/bj1020216 PMC Full Text View journal details

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The action of cabbage-leaf phospholipase D upon lysolecithin.

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