Antibiotic control of Mycoplasma in tissue culture.

Applied microbiology ·Vol. 15 ·No. 1 ·1967-01-00 ·Pages 82-5

Perlman D, Rahman SB, Semar JB

Abstract

Seven of eight strains of Mycoplasma (PPLO) were found to be sensitive to the deoxystreptamines, certain macrolides, and the tetracyclines. These antibiotics are relative noncytotoxic. Kanamycin and tetracycline were useful in eliminating PPLO (pleuropneumonia-like organisms) strain Squibb no. 1 from a HeLa cell line which was deliberately contaminated with PPLO. Repeated exposure of M. laidlawii type B cells to neomycin resulted in a 50-fold increase in resistance, and the resistant strain was also resistant to gentamicin, kanamycin, neomycin, and paromomycin. A tetracycline-resistant strain of this culture was found to be resistant to 7-chlortetracycline, 7-chlor-6-demethyltetracycline, and 5-hydroxytetracycline. One PPLO strain, Squibb no. 2, derived from a contaminated HeLa cell culture, was resistant to all antibiotics studied.

MeSH Terms

Anti-Bacterial Agents/pharmacology Drug Resistance, Microbial HeLa Cells Humans Mycoplasma/drug effects Tetracycline/pharmacology

Chemicals

Anti-Bacterial Agents Tetracycline

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Perlman D

Rahman S B

Semar J B

References (15)

15 references, click to expand

Decontamination of pleuropneumonia-like organism (PPLO) infected tissue cultures.
Experientia. 1964 Nov 15;20(11):651-2 PMID: 4285557
Antibiotic effect of tylosin on a mycoplasma contaminant in a tissue culture leukemia cell line.
Proc Soc Exp Biol Med. 1966 Apr;121(4):1009-10 PMID: 4287117
Effect of antibiotics on growth of Mycoplasma pneumoniae Mac.
J Antibiot (Tokyo). 1966 May;19(3):118-20 PMID: 6012893
Pleuropneumonia-like (mycoplasma) infections of tissue culture.
J Bacteriol. 1961 Apr;81:626-35 PMID: 13691094
Elimination of pleuropneumonia-like organisms from cell cultures.
Exp Cell Res. 1960 Oct;21:242-4 PMID: 13700471
Isolation and elimination of pleuropneumonia-like organisms from mammalian cell cultures.
Proc Soc Exp Biol Med. 1960 Oct;105:10-5 PMID: 13736879
Mammalian cell cultures contaminated with PPLO III. Elimination of PPLO with specific antiserum.
Proc Soc Exp Biol Med. 1963 Jan;112:176-81 PMID: 13972030
Experimental infection of tissue cultures with certain mycoplasma (PPLO).
Proc Soc Exp Biol Med. 1963 Mar;112:643-7 PMID: 14019261
Mammalian cell cultures contaminated with pleuropneumonia-like organisms. I. Effect of pleuropneumonia-like organisms on growth of established cell strains.
J Infect Dis. 1963 Jan-Feb;112:7-16 PMID: 14031982
MAMMALIAN CELL CULTURES CONTAMINATED WITH PLEUROPNEUMONIA-LIKE ORGANISMS. II. EFFECT OF PPLO ON CELL MORPHOLOGY IN ESTABLISHED MONOLAYER CULTURES.
Exp Cell Res. 1963 Aug;31:321-8 PMID: 14056991
ELIMINATION OF PLEUROPNEUMONIA-LIKE ORGANISMS FROM EMBRYONIC HUMAN LUNG TISSUE CULTURE WITH TETRACYCLINE.
J Bacteriol. 1964 Jan;87:237 PMID: 14102867
A METHOD FOR ERADICATION OF MYCOPLASMA INFECTIONS IN CELL CULTURES.
Proc Soc Exp Biol Med. 1964 Dec;117:918-21 PMID: 14244989
Effects of antibiotics on multiplication of L cells in suspension culture.
Proc Soc Exp Biol Med. 1959 Nov;102:290-2 PMID: 14431890
Decontaminating tissue cultures infected with pleuropneumonia-like organisms.
Nature. 1960 Mar 12;185:783-4 PMID: 14400339
Detection, elimination, and prevention of contamination of cell cultures with pleuropneumonialike organisms.
J Bacteriol. 1959 Oct;78:575-82 PMID: 14400425

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.15.1.82-85.1967

Published

1967-01-00

Pages

82-5

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC546847

Subset

External Links

PubMed source DOI: 10.1128/am.15.1.82-85.1967 PMC Full Text

Analysis Services

Antibiotic control of Mycoplasma in tissue culture.

Contact

WeChat Official Account