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PMID: 6032982 Published · ppublish English Journal Article

A simple method for the isolation of adrenal chromaffin granules on a large scale.

The Biochemical journal ·Vol. 103 ·No. 2 ·1967-05-00 ·Pages 480-2

Smith AD, Winkler H

Abstract

Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1.6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes.

MeSH Terms
Adenosine Triphosphate/analysis Adrenal Medulla/cytology Animals Catecholamines/analysis Cattle Chromaffin System/analysis Deoxyribonucleases/analysis Fluorometry Horses Hydro-Lyases/analysis Nitrogen/analysis Rats Swine Ultracentrifugation
Chemicals
Catecholamines Adenosine Triphosphate Deoxyribonucleases Hydro-Lyases Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith A D
Winkler H
References (11)
11 references, click to expand
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    J Physiol. 1958 Apr 3;141(1):183-92 PMID: 13539831
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  7. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.
    Biochim Biophys Acta. 1950 Jan;4(1-3):211-4 PMID: 15403927
  8. Determination of ATP and related compounds: firefly luminescence and other methods.
    Methods Biochem Anal. 1954;1:341-56 PMID: 13193535
  9. Purification and properties of an acidic protein from chromaffin granules of bovine adrenal medulla.
    Biochem J. 1967 May;103(2):483-92 PMID: 4962084
  10. The localization of lysosomal enzymes in chromaffin tissue.
    J Physiol. 1966 Mar;183(1):179-88 PMID: 5945247
  11. Mitochondrial enzymes and chromaffin granules.
    J Physiol. 1957 Dec 3;139(2):316-22 PMID: 13492215
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030480
Published
1967-05-00
Pages
480-2
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270431
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030480 PMC Full Text View journal details
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