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PMID: 6033763 Published · ppublish English Journal Article

Enzymes in cancer. Asparaginase from chicken liver.

The Biochemical journal ·Vol. 103 ·No. 1 ·1967-04-00 ·Pages 238-45

Ohnuma T, Bergel F, Bray RC

Abstract

1. A procedure for partial purification of asparaginase from chicken liver is presented. 2. The bulk of the enzyme is located in the soluble fraction of chicken liver. 3. Molecular weights of chicken-liver asparaginase and of the guinea-pig serum enzyme, estimated by gel filtration, were 306000 and 210000 respectively. The Michaelis constants (K(m)) at 37 degrees and pH8.5 were 6.0x10(-5)m and 7.2x10(-5)m respectively. 4. At 50 degrees the chicken-liver enzyme was moderately stable, some activity being lost by aggregation; in dilute electrolyte solutions the activity rapidly diminished. 5. The anti-lymphoma effect of guinea-pig serum in mice carrying the 6C3HED tumour was confirmed. Chicken-liver asparaginase also showed an effect but in this case the enzyme preparation had to be administered repeatedly. 6. Guinea-pig serum asparaginase was stable for several days in mouse blood, after intraperitoneal injection, whereas chicken-liver asparaginase rapidly disappeared. 7. Aspartic acid beta-hydrazide was shown to be a competitive inhibitor of chicken-liver asparaginase with K(i) approx. 5.6x10(-4)m. In mice it produced an anti-lymphoma effect, as reported previously.

MeSH Terms
Amidohydrolases/analysis,therapeutic use Animals Aspartic Acid/pharmacology Cattle Chromatography, Gel Enzymes/blood Guinea Pigs Hydrazines/pharmacology Kinetics Liver/enzymology Lymphoma/drug therapy Mice Molecular Weight Neoplasms, Experimental/drug therapy Poultry Rabbits Rats
Chemicals
Enzymes Hydrazines Aspartic Acid Amidohydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ohnuma T
Bergel F
Bray R C
References (29)
29 references, click to expand
  1. The chemistry of xanthine oxidase. 9. An improved method of preparing the bovine milk enzyme.
    Biochem J. 1964 Feb;90(2):350-3 PMID: 5834246
  2. [Direct determination of ammonium in blood and tissue extracts by means of the phenol by chlorite reaction].
    Clin Chim Acta. 1963 Jan;8:5-11 PMID: 14034603
  3. Inhibitory effect of guinea pig serum on a number of new leukaemias in mice.
    Nature. 1963 May 25;198:800 PMID: 14014746
  4. In vivo and in vitro studies of the action of guinea pig serum against the ascites form of the MurphySturm lymphosarcoma.
    Cancer Res. 1958 Dec;18(11):1309-13 PMID: 13608438
  5. TUMOR INHIBITORY EFFECT OF L-ASPARAGINASE FROM ESCHERICHIA COLI.
    Arch Biochem Biophys. 1964 May;105:450-2 PMID: 14186753
  6. Antilymphoma activity of L-asparaginase in vivo: clearance rates of enzyme preparations from guinea pig serum and yeast in relation to their effect on tumor growth.
    J Natl Cancer Inst. 1965 Dec;35(6):967-74 PMID: 5856684
  7. A steam distillation apparatus suitable for micro-Kjeldahl analysis.
    Biochem J. 1942 Dec;36(10-12):790-1 PMID: 16747508
  8. Effects of certain amino acid derivatives on microorganisms and sarcoma 180.
    J Natl Cancer Inst. 1958 Mar;20(3):495-512 PMID: 13539603
  9. Action of guinea pig serum and human gamma globulin on the growth of a rat tumor.
    Science. 1956 Nov 16;124(3229):980-1 PMID: 13380410
  10. Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum. II. Studies on the nature of the active serum constituent: histological mechanism of the regression: tests for effects of guinea pig serum on lymphoma cells in vitro: discussion.
    J Exp Med. 1953 Dec;98(6):583-606 PMID: 13109111
  11. Evidence that the L-asparaginase of guinea pig serum is responsible for its antilymphoma effects. I. Properties of the L-asparaginase of guinea pig serum in relation to those of the antilymphoma substance.
    J Exp Med. 1963 Jul;118:99-120 PMID: 14015821
  12. GUINEA PIG SERUM AND LIVER ASPARAGINASES. PURIFICATION AND ANTITUMOR ACTIVITY.
    J Biol Chem. 1965 May;240:2234-41 PMID: 14299653
  13. Studies on the free amino acids and related compounds in the tissues of the cat.
    J Biol Chem. 1954 Dec;211(2):927-39 PMID: 13221598
  14. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.
    Biochem J. 1955 Aug;60(4):604-17 PMID: 13249955
  15. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.
    Biochem J. 1965 Sep;96(3):595-606 PMID: 5862401
  16. Evidence that the L-asparaginase of guinea pig serum is responsible for its antilymphoma effects. II. Lymphoma 6C3HED cells cultured in a medium devoid of L-asparagine lose their susceptibility to the effects of guinea pig serum in vivo.
    J Exp Med. 1963 Jul;118:121-48 PMID: 14015822
  17. The inhibition by guinea pig serum of the growth of the Murphy-Sturm lymphosarcoma.
    Cancer Res. 1958 Aug;18(7):866-8 PMID: 13573356
  18. Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum. I. Course of transplanted cancers of various kinds in mice and rats given guinea pig serum, horse serum, or rabbit serum.
    J Exp Med. 1953 Dec;98(6):565-82 PMID: 13109110
  19. Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells.
    J Biol Chem. 1959 May;234(5):1029-35 PMID: 13654313
  20. Hydrolysis and transfer reactions catalyzed by omega-amidase preparations.
    J Biol Chem. 1955 Jul;215(1):441-60 PMID: 14392177
  21. The L-amino acid oxidases of snake venom. II. Isolation and characterization of homogeneous L-amino acid oxidase.
    Arch Biochem. 1950 Nov;29(1):190-209 PMID: 14790807
  22. The biosynthesis of dicarboxylic amino acids and enzymic transformations of amides in plants.
    Adv Enzymol Relat Subj Biochem. 1958;20:319-40 PMID: 13605987
  23. The free amino acids of human blood plasma.
    J Biol Chem. 1954 Dec;211(2):915-26 PMID: 13221597
  24. The chemistry of xanthine oxidase. The problems of enzyme inactivation and stabilization.
    Biochem J. 1959 Sep;73:182-92 PMID: 13799274
  25. Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver.
    Biochem J. 1955 Mar;59(3):438-45 PMID: 14363114
  26. The effects of components of guinea pig serum on lymphosarcoma 6C3HED in C3H mice.
    Blood. 1958 Aug;13(8):732-9 PMID: 13560571
  27. The asparagine deamidase of Bacillus coagulans and Bacillus stearothermophilus.
    Can J Microbiol. 1957 Dec;3(7):1001-9 PMID: 13489550
  28. Guinea pig serum L-asparaginase. Properties, purification, and application to determination of asparagine in biological samples.
    J Biol Chem. 1963 Mar;238:983-93 PMID: 13993876
  29. Antagonism of purified asparaginase from guinea pig serum toward lymphoma.
    Science. 1966 Feb 25;151(3713):998-9 PMID: 5952048
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030238
Published
1967-04-00
Pages
238-45
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270391
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030238 PMC Full Text View journal details
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