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PMID: 6035517 Published · ppublish English Journal Article

The exchange of unesterified cholesterol between human low-density lipoproteins and rat erythrocyte 'ghosts'.

The Biochemical journal ·Vol. 104 ·No. 1 ·1967-07-00 ·Pages 270-7

Bruckdorfer KR, Green C

Abstract

1. The exchange of unesterified cholesterol molecules between rat erythrocyte ;ghosts' and human low-density lipoproteins has been studied under a number of different experimental conditions. 2. The process is pH-dependent, the rate being minimal at about pH5. 3. Cholesterol exchange does not vary greatly with temperature, the rate at 50 degrees being less than twice that at 2 degrees . 4. Large variations in the ionic strength or Ca(2+) concentration of the medium have little effect, but the exchange rate is greatly increased in the presence of a wide range of chemical compounds, e.g. urea, alcohols, acetone, dimethyl sulphoxide and tetra-alkyl-ammonium salts. 5. Acetone and dimethyl sulphoxide have a much greater effect at 37 degrees than at 8-10 degrees . 6. It is proposed that hydrophobic bonding is of great importance in maintaining the structure of ;ghosts' and lipoproteins. 7. The results are discussed in relation to current theories of membrane and lipoprotein structure.

MeSH Terms
Acetone/pharmacology Animals Cell Membrane/metabolism Cholesterol/metabolism Dimethyl Sulfoxide/pharmacology Erythrocytes/cytology,metabolism Hydrogen-Ion Concentration In Vitro Techniques Lipoproteins/metabolism Phospholipases Rats Temperature
Chemicals
Lipoproteins Acetone Cholesterol Phospholipases Dimethyl Sulfoxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bruckdorfer K R
Green C
References (33)
33 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1040270
Published
1967-07-00
Pages
270-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270572
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1040270 PMC Full Text View journal details
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