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PMID: 604729 Published · ppublish English Journal Article

Salt-starch xylose lysine deoxycholate agar. A single medium for the isolation of sodium and non-sodium dependent enteric gram-negative bacilli.

Medical microbiology and immunology ·Vol. 163 ·No. 4 ·1977-12-27 ·Pages 241-9

Roland FP

Abstract

Salt dependent gram-negative bacilli responsible for gastroenteritis and tissue infections are often not recovered because proper media for isolation are not used. A salt-starch XLD agar with 1.5% NaC1 and 0.5% starch medium has been found to permit the isolation of pathogenic Enterobacteriaceae, non-Enterobacteriaceae gram-negative bacilli, and salt-dependent gram-negative bacilli, among which is Vibrio parahemolyticus. As far as the Enterobacteriaceae are concerned, the selectivity and sensitivity of the medium are the same as with standard media with the added advantage of isolating salt-dependent organisms, thereby saving time and money. It can be used for routine blood cultures, investigation of sea water, seafood and tissue infections related to marine activities.

MeSH Terms
Agar Bacteria/isolation & purification Bacterial Infections/diagnosis Deoxycholic Acid Diagnosis, Differential Enterobacteriaceae/isolation & purification Feces/microbiology Humans Lysine Skin/microbiology Sodium Chloride Species Specificity Starch Vibrio parahaemolyticus/isolation & purification Xylose
Chemicals
Deoxycholic Acid Sodium Chloride Agar Starch Xylose Lysine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Roland F P
References (13)
13 references, click to expand
  1. Vibrio parahemolyticus in Narragansett Bay.
    R I Med J. 1973 Aug;56(8):321-4 PMID: 4516955
  2. Isolations of organisms related to Vibrio parahemolyticus from American estuarine sediments.
    Appl Microbiol. 1968 Mar;16(3):543-6 PMID: 5649873
  3. Leg gangrene and endotoxin shock due to vibrio parahaemolyticus--an infection acquired in New England coastal waters.
    N Engl J Med. 1970 Jun 4;282(23):1306 PMID: 5442360
  4. Vibrio parahaemolyticus outbreaks in the United States.
    Lancet. 1974 Mar 30;1(7857):551-4 PMID: 4131985
  5. Isolation of Vibrio parahaemolyticus from fecal specimens on mannitol salt agar.
    J Clin Microbiol. 1976 Aug;4(2):175-9 PMID: 965479
  6. Vibrio parahaemolyticus: a potential pathogen from marine sources in Britain.
    Lancet. 1972 Feb 26;1(7748):485-6 PMID: 4109828
  7. Modified selective and differential isolation medium for Vibrio parahaemolyticus.
    Appl Microbiol. 1971 Oct;22(4):593-9 PMID: 4943272
  8. Procedure for isolation and enumeration of Vibrio parahaemolyticus.
    Appl Microbiol. 1972 Jan;23(1):26-33 PMID: 4333897
  9. The numbers of pathogenic bacilli in faeces in intestinal diseases.
    J Hyg (Lond). 1955 Jun;53(2):217-24 PMID: 14392319
  10. Fulminating vibrio parahemolyticus septicemia. A syndrome of erythemia multiforme, hemolytic anemia, and hypotension.
    Arch Intern Med. 1974 Mar;133(3):479-81 PMID: 4811486
  11. Tissue invasion by unnamed marine vibrios.
    JAMA. 1975 Sep 15;233(11):1173-6 PMID: 240041
  12. Letter: Vibrio parahaemolyticus.
    Lancet. 1974 Jun 8;1(7867):1171 PMID: 4137031
  13. Isolation of vibrio parahaemolyticus from the Northwest Pacific.
    Nature. 1968 Mar 30;217(5135):1263-4 PMID: 5643107
Article Info
Journal
Medical microbiology and immunology
Abbr.
Med Microbiol Immunol
ISSN
0300-8584
Published
1977-12-27
Pages
241-9
Language
English
Region
Germany
NLM ID
0314524
Subset
IM
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