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PMID: 6084811 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of monoclonal antibodies specific for products of avian oncogene myb.

Molecular and cellular biology ·Vol. 4 ·No. 12 ·1984-12-00 ·Pages 2843-50

Evan GI, Lewis GK, Bishop JM

Abstract

We isolated a series of monoclonal antibodies which were raised against a bacterially expressed protein, bp37v-myb, and coded for by part of the avian v-myb gene. These monoclonal antibodies recognized a range of antigenic specificities on bp37v-myb, and this was reflected in their differing specificities for the gene products of the v-myb, c-myb, and E26 viral oncogenes. One monoclonal antibody recognized, in addition to the v-myb and c-myb gene products, a conserved nuclear protein found in all tested cells. We describe the characterization of these monoclonal antibodies.

MeSH Terms
Animals Antibodies, Monoclonal/isolation & purification Antibody Specificity Cell Line Electrophoresis, Polyacrylamide Gel Epitopes/analysis Oncogenes Viral Proteins/immunology
Chemicals
Antibodies, Monoclonal Epitopes Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Evan G I
Lewis G K
Bishop J M
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20 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.12.2843-2850.1984
Published
1984-12-00
Pages
2843-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369296
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.12.2843-2850.1984 PMC Full Text View journal details
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