Home LiteratureArticle Details

PMID: 608686 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro inhibition of human peripheral blood lymphocyte transformation by an extract of Pseudomonas putida.

Immunology ·Vol. 33 ·No. 2 ·1977-08-00 ·Pages 209-15

Hunt CV, Khan W, Friedman G, Houck JC

Abstract

An extract prepared from a psychrophilic strain of Pseudomonas putida was found to cause a dose-dependent inhibition of [H3]TdR incorporation into human peripheral blood lymphocytes stimulated with PHA, ConA, PWM, or in a mixed lymphocyte reaction. The inhibition was found not to be the result of cytotoxicity, culture medium depletion of a component necessary for lymphocyte transformation, or interference with label uptake by blast lymphocytes. The extract was most effective when added prior to 48 h of mitogen stimulation. The inhibitory material was photeolytic enzyme degradable, heat-stable and non-dialyzable.

MeSH Terms

Cell Extracts/pharmacology Culture Media Cytotoxicity Tests, Immunologic Humans Kinetics Lymphocyte Activation Pseudomonas Thymidine/metabolism Tissue Extracts/pharmacology

Chemicals

Cell Extracts Culture Media Tissue Extracts Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hunt C V

Khan W

Friedman G

Houck J C

References (15)

15 references, click to expand

PSYCHROPHILIC BACTERIA.
Bacteriol Rev. 1959 Sep;23(3):97-108 PMID: 16350157
The modulating effect of cholera enterotoxin on the immune response.
J Immunol. 1974 Sep;113(3):729-39 PMID: 4370486
L-asparaginase and blastogenesis.
Lancet. 1969 Feb 22;1(7591):423 PMID: 4179271
Studies of PPLO infection. V. Inhibition of lymphocyte mitosis and antibody formation by mycoplasmal extracts.
J Exp Med. 1969 Jun 1;129(6):1163-81 PMID: 4306341
Lymphocyte transformation and macrophage migration inhibition by electrofocused and gel-filtered fractions of group A streptococcal filtrate.
Cell Immunol. 1974 Dec;14(3):366-75 PMID: 4619355
Pathophysiologic effects of lethal and immunoregulatory doses of cholera enterotoxin in the mouse.
J Immunol. 1974 Sep;113(3):740-9 PMID: 4606891
The differential effect of cholera toxin on the lymphocyte stimulation induced by various mitogens.
Immunology. 1975 Aug;29(2):275-82 PMID: 169198
Modulation of in vivo antibody responses by cholera toxin.
J Immunol. 1975 Jan;114(1 Pt 1):81-4 PMID: 163279
Inhibition of mitogen stimulation of human peripheral blood leukocytes by Vibrio cholerae enterotoxin.
J Immunol. 1975 Jan;114(1 Pt 2):476-80 PMID: 123258
A potent antimitogenic factor from group A streptococci.
Immunology. 1975 Jan;28(1):103-12 PMID: 123216
Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesis.
Exp Cell Res. 1973 Mar 30;78(1):201-8 PMID: 4690921
Immunosuppression by endotoxin and its lipoid A component.
Proc Soc Exp Biol Med. 1967 Mar;124(3):919-24 PMID: 4960833
Immunosuppressive effect of cell-free extracts from Escherichia coli.
Transplant Proc. 1971 Mar;3(1):831-4 PMID: 4937978
Delayed rejection of skin homografts in Pseudomonas sepsis.
Surg Gynecol Obstet. 1967 May;124(5):1067-70 PMID: 5336870
Immunosuppressant from group A streptococci.
Science. 1968 Feb 23;159(3817):880-1 PMID: 5636328

Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1977-08-00

Pages

209-15

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1445343

Subset

External Links

PubMed source PMC Full Text View journal details

Analysis Services

In vitro inhibition of human peripheral blood lymphocyte transformation by an extract of Pseudomonas putida.

Contact

WeChat Official Account