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PMID: 6087314 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 14 ·1984-07-00 ·Pages 4255-9

Linzer DI, Nathans D

Abstract

As part of the proliferative response to serum, mouse 3T3 cells produce a set of growth-related mRNAs identified by hybridization to cloned cDNAs. One of these mRNAs, which is about 1 kilobase long, appears within a few hours after stimulation of resting cells with serum or platelet-derived growth factor and reaches a high level during the transition from the G1 to the S phase of growth. This mRNA is translated in vitro into a protein of approximately 25 kilodaltons. The corresponding cloned cDNA of 791 base pairs has been sequenced; it contains a single open reading frame that encodes a protein of 224 amino acids with extensive sequence homology to mammalian prolactins. The initial 29-amino acid segment of the encoded protein resembles the signal sequences of prehormones. That the growth-related protein is not mouse prolactin is indicated by comparison of its predicted amino acid composition with that of mouse prolactin and by the distinct fragment patterns seen when restricted mouse DNA is probed with the cloned cDNA or rat prolactin cDNA. Therefore, the growth-related protein appears to be a new member of the prolactin-growth hormone family. Because of its relationship to prolactin and growth hormone and its association with cell proliferation, the protein has been called "proliferin."

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA/analysis DNA Restriction Enzymes/metabolism Glycoproteins Growth Hormone/analysis,genetics Intercellular Signaling Peptides and Proteins Mice Mice, Inbred BALB C Prolactin/analysis,genetics Proteins/genetics RNA, Messenger/analysis
Chemicals
Glycoproteins Intercellular Signaling Peptides and Proteins Prl2c2 protein, mouse Proteins RNA, Messenger Prolactin Growth Hormone DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Linzer D I
Nathans D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.14.4255
Published
1984-07-00
Pages
4255-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345566
Subset
IM
Grants
NCI NIH HHS · 5 PO1 CA16519 · United States
Databases
GENBANK
K02245
External Links
PubMed source DOI: 10.1073/pnas.81.14.4255 PMC Full Text View journal details
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