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PMID: 6087315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Avian myeloblastosis virus and E26 virus oncogene products are nuclear proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 14 ·1984-07-00 ·Pages 4265-9

Boyle WJ, Lampert MA, Lipsick JS, Baluda MA

Abstract

The defective acute leukemia viruses avian myeloblastosis virus (AMV) and E26 virus each contain an inserted cellular sequence related to the same highly conserved cellular gene, proto-amv. The oncogenes of these two retroviruses differ from this cellular proto-oncogene in gene structure, transcript structure, and gene product. The product of the AMV oncogene (myb) is a 48,000 Mr protein, p48myb, encoded by a transduced segment (amv) of proto-amv flanked by short helper-virus-derived terminal sequences. The E26 virus oncogene product is a 135,000 Mr protein, p135gag-amve-ets, encoded by significant portions of a viral structural gene (gag), sequences related to proto-amv (amve), and additional E26-specific sequences (ets) transduced from cellular proto-ets. Both p48myb and p135gag-amve-ets transforming proteins are located in the nucleus of cells transformed by these viruses. A protein of 110,000 Mr which is specifically immunoprecipitated by antisera to amv peptides and may be the product of the normal cellular gene (proto-amv) has been located in the cytoplasm of cells that express proto-amv mRNA.

MeSH Terms
Animals Avian Leukosis Virus/genetics Avian Myeloblastosis Virus/genetics Cell Line Chickens Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Molecular Weight Oncogenes Peptides/analysis Transduction, Genetic Transforming Growth Factors Viral Proteins/analysis
Chemicals
Peptides Viral Proteins Transforming Growth Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boyle W J
Lampert M A
Lipsick J S
Baluda M A
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.14.4265
Published
1984-07-00
Pages
4265-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345568
Subset
IM
Grants
NCI NIH HHS · CA-09030 · United States
NCI NIH HHS · CA-10197 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.14.4265 PMC Full Text View journal details
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