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PMID: 6087343 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence 5' of the chicken c-myc coding region: localization of a noncoding exon that is absent from myc transcripts in most avian leukosis virus-induced lymphomas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 15 ·1984-08-00 ·Pages 4697-701

Shih CK, Linial M, Goodenow MM, Hayward WS

Abstract

We have determined the nucleotide sequence of the 2.2-kilobase-pair region upstream of the chicken c-myc coding exons. Using RNA blot analysis, we have localized a noncoding exon to a region that is separated from the c-myc coding sequences by an intron of 700-800 base pairs. In most avian leukosis virus-induced lymphomas proviral integration has occurred within, or downstream of, the first exon, thus presumably displacing the regulatory sequences that normally control c-myc expression. More than 70% of the integration sites were clustered in a 250-base-pair region in the first intron, immediately preceding the coding sequences. Sequences from the upstream noncoding exon were absent from the myc transcripts in these lymphomas; RNA transcripts from the normal c-myc allele were not expressed at detectable levels.

MeSH Terms
Animals Avian Leukosis Virus/genetics Base Sequence Cell Transformation, Viral Chickens Genes Lymphoma/genetics,microbiology Oncogenes RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shih C K
Linial M
Goodenow M M
Hayward W S
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.15.4697
Published
1984-08-00
Pages
4697-701
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391557
Subset
IM
Grants
NCI NIH HHS · CA18282 · United States
NCI NIH HHS · CA34502 · United States
Databases
GENBANK
J00889
External Links
PubMed source DOI: 10.1073/pnas.81.15.4697 PMC Full Text View journal details
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