Home LiteratureArticle Details
PMID: 6089106 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of endonuclease J: a sequence-specific endonuclease cleaving immunoglobulin genes.

Nucleic acids research ·Vol. 12 ·No. 15 ·1984-08-10 ·Pages 5995-6010

Kataoka T, Kondo S, Nishi M, Kodaira M, Honjo T

Abstract

An endonuclease activity which cleaves close to the recombination sites of the immunoglobulin JK segments was found in extracts of chicken bursa of Fabricius and characterized after partial purification. The enzyme preparation also cleaved a VK segment at its 3' end. A similar activity was found in mouse liver, mouse myelomas and Hela cells. The enzyme designated as endonuclease J introduces double-stranded cleavages preferentially at sequences containing G clusters of pBR322 as well as the JK segments. However, not all the G clusters were cleaved by endonuclease J, suggesting that the enzyme recognizes additional sequences. Deletion of the conserved nonamer (GGTTTTTGT) located immediately 5' to the JK4 segment drastically reduced the cleavage activity of its immediate downstream G cluster. Although biological function of endonuclease J is not clear at this stage, the possibilities of its involvement in the immunoglobulin gene recombination and general recombination were discussed.

MeSH Terms
Animals Antibody Diversity Base Sequence Bursa of Fabricius/enzymology Chickens Endodeoxyribonucleases/isolation & purification,metabolism Endonucleases/isolation & purification Immunoglobulin Constant Regions/genetics Immunoglobulin kappa-Chains/genetics Immunoglobulins/genetics Recombination, Genetic Substrate Specificity
Chemicals
Immunoglobulin Constant Regions Immunoglobulin kappa-Chains Immunoglobulins Endodeoxyribonucleases Endonucleases endonuclease J
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kataoka T
Kondo S
Nishi M
Kodaira M
Honjo T
References (32)
32 references, click to expand
  1. Organization of immunoglobulin heavy chain genes and allelic deletion model.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2140-4 PMID: 97665
  2. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  3. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  4. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  5. A kappa-immunoglobulin gene is formed by site-specific recombination without further somatic mutation.
    Nature. 1979 Aug 2;280(5721):370-5 PMID: 111146
  6. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  7. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):955-61 PMID: 4909471
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Cloning immunoglobulin gamma 2b chain gene of mouse: characterization and partial sequence determination.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4240-4 PMID: 116231
  10. An immunoglobulin heavy-chain gene is formed by at least two recombinational events.
    Nature. 1980 Feb 21;283(5749):733-9 PMID: 6766532
  11. Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):919-23 PMID: 6767246
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  14. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  15. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  16. The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72 PMID: 6256738
  17. Cruciform structures in supercoiled DNA.
    Nature. 1981 Feb 5;289(5797):466-70 PMID: 7464915
  18. Repetitive sequences in class-switch recombination regions of immunoglobulin heavy chain genes.
    Cell. 1981 Feb;23(2):357-68 PMID: 6781756
  19. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids.
    Gene. 1981 Oct;15(1):99-102 PMID: 6271634
  20. The nucleotide sequences of rearranged and germline immunoglobulin VH genes of a mouse myeloma MC101 and evolution of VH genes in mouse.
    J Biol Chem. 1982 Jan 10;257(1):277-85 PMID: 6273429
  21. Recombined flanks of the variable and joining segments of immunoglobulin genes.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1383-7 PMID: 6803241
  22. DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):262-6 PMID: 6804938
  23. Nucleotide sequences of switch regions of immunoglobulin C epsilon and C gamma genes and their comparison.
    J Biol Chem. 1982 Jul 10;257(13):7322-9 PMID: 6282840
  24. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4118-22 PMID: 6287467
  25. Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus.
    Cell. 1982 Oct;30(3):807-16 PMID: 6291785
  26. A novel type of aberrant recombination in immunoglobulin genes and its implications for V-J joining mechanism.
    Nature. 1983 Mar 17-23;302(5905):260-3 PMID: 6403866
  27. Escherichia coli extract-catalyzed recombination in switch regions of mouse immunoglobulin genes.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2666-70 PMID: 6221341
  28. Chi hotspots of generalized recombination.
    Cell. 1983 Oct;34(3):709-10 PMID: 6354468
  29. The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
    Nucleic Acids Res. 1983 Nov 11;11(21):7303-16 PMID: 6316256
  30. Joining of V kappa to J kappa gene segments in a retroviral vector introduced into lymphoid cells.
    Nature. 1984 Mar 29-Apr 4;308(5958):425-8 PMID: 6323991
  31. Double-stranded cleavage by cell extracts near recombinational signal sequences of immunoglobulin genes.
    Nature. 1984 Apr 26-May 2;308(5962):860-2 PMID: 6325923
  32. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.15.5995
Published
1984-08-10
Pages
5995-6010
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320052
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.15.5995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2