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PMID: 6089173 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The role of a signaling protein in bacterial sensing: behavioral effects of increased gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 16 ·1984-08-00 ·Pages 5056-60

Clegg DO, Koshland DE

Abstract

A recombinant DNA approach has been used to study intracellular signaling in the bacterial sensing system. The Escherichia coli cheY gene, whose function is unknown, has been subcloned behind the synthetic inducible tac promoter. The resulting plasmid directs the synthesis of the Y protein in response to isopropyl beta-D-thiogalactoside, independent of its usual operon control. When this construct was introduced into wild-type and mutant cells, the Y protein caused a clockwise rotational bias in the flagellar motors. This effect was observed even in heavily biased counterclockwise strains lacking most of the central chemotaxis processing genes. The results show that the Y protein has a direct influence on flagellar rotation not requiring other processing genes of the sensing system. The Y protein appears to bind directly to a part of the flagellar motor, probably the flaA gene product, and it is probably the key element in biasing the motor toward the clockwise rotational direction.

MeSH Terms
Base Sequence Cell Movement DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/genetics,physiology Genes, Bacterial Peptides/metabolism Plasmids Protein Sorting Signals
Chemicals
DNA, Recombinant Peptides Protein Sorting Signals DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clegg D O
Koshland D E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.16.5056
Published
1984-08-00
Pages
5056-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391636
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.16.5056 PMC Full Text View journal details
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