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PMID: 6089198 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The structure of the human tissue-type plasminogen activator gene: correlation of intron and exon structures to functional and structural domains.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 17 ·1984-09-00 ·Pages 5355-9

Ny T, Elgh F, Lund B

Abstract

A genomic clone carrying the human tissue-type plasminogen activator (t-PA) gene was isolated from a cosmid library, and the gene structure was elucidated by restriction mapping, Southern blotting, and DNA sequencing. The cosmid contained all the coding parts of the mRNA, except for the first 58 bases in the 5' end of the mRNA, and had a total length of greater than 20 kilobases. It was separated into at least 14 exons by at least 13 introns, and the exons seemed to code for structural or functional domains. Thus, the signal peptide, the propeptide, and the domains of the heavy chain, including the regions homologous to growth factors, and to the "finger" structure of fibronectin, are all encoded by separate exons. In addition, the two kringle regions of t-PA were both coded for by two exons and were cleaved by introns at identical positions. The region coding for the light chain, comprising the serine protease part of the molecule was split by four introns, revealing a gene organization similar to other serine proteases.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes Genes Humans Plasmids Plasminogen Activators/genetics Protein Conformation
Chemicals
DNA DNA Restriction Enzymes Plasminogen Activators
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ny T
Elgh F
Lund B
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.17.5355
Published
1984-09-00
Pages
5355-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391702
Subset
IM
Databases
GENBANK
J00278, L00141, L00142, L00143, L00144, L00145, L00146, L00147, L00148, L00149, L00150, L00151, L00152, L00153
External Links
PubMed source DOI: 10.1073/pnas.81.17.5355 PMC Full Text View journal details
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