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PMID: 6089212 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Clones from the human gene complex coding for salivary proline-rich proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 17 ·1984-09-00 ·Pages 5561-5

Azen E, Lyons KM, McGonigal T, Barrett NL, Clements LS, Maeda N, Vanin EF, Carlson DM, Smithies O

Abstract

The salivary protein gene complex consists of a series of loci coding for related but distinct proline-rich proteins (PRPs) found chiefly in saliva. We have screened a library of human genomic DNA fragments in bacteriophage lambda Charon 4A with a PRP cDNA synthesized and cloned from rat parotid gland mRNA. Two phages (PRP1 and PRP2) hybridizing to the rat probe under moderately stringent conditions contain related but not identical DNAs. Preliminary nucleotide sequence data indicate that both DNAs include regions comprised of nearly identical tandemly repeated sequences, each able to code for about 21 amino acids. The decoded consensus repeat sequence is homologous to the repeating amino acid units found by others in human PRPs. This and other features demonstrate that these two clones are members of the PRP gene family. Polymorphic differences between the DNAs of different individuals were observed after probing digests of human genomic DNA with a HinfI fragment from PRP1. These DNA polymorphisms reflect size differences, possibly caused by frequent unequal crossing-over between the repeated units in the PRP genes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Humans Nucleic Acid Hybridization Parotid Gland/metabolism Peptides/genetics Proline-Rich Protein Domains RNA, Messenger/genetics Rats Salivary Proteins and Peptides/genetics
Chemicals
Peptides RNA, Messenger Salivary Proteins and Peptides DNA DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Azen E
Lyons K M
McGonigal T
Barrett N L
Clements L S
Maeda N
Vanin E F
Carlson D M
Smithies O
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.17.5561
Published
1984-09-00
Pages
5561-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391746
Subset
IM
Grants
NIADDK NIH HHS · AM 19175 · United States
NIDCR NIH HHS · DEO 3658-19 · United States
NIGMS NIH HHS · GM 20069 · United States
Databases
GENBANK
K02575, K02576, K02577, K02578
External Links
PubMed source DOI: 10.1073/pnas.81.17.5561 PMC Full Text View journal details
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