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PMID: 6089340 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Infectious and selectable retrovirus containing an inducible rat growth hormone minigene.

Science (New York, N.Y.) ·Vol. 225 ·No. 4666 ·1984-09-07 ·Pages 993-8

Miller AD, Ong ES, Rosenfeld MG, Verma IM, Evans RM

Abstract

A growth hormone minigene carrying its natural promoter (237 nucleotides of chromosomal DNA) was stably propagated in a murine retrovirus containing hypoxanthine-guanine phosphoribosyltransferase as a selectable marker. Glucocorticoid and thyroid hormone inducibility was transferred with the growth hormone gene. Recombinant virus with titers of 10(6) per milliliter was recovered. This demonstration that retroviruses can be used to transfer a nonselectable gene under its own regulatory control enlarges the scope of retroviral vectors as potent tools for gene transfer.

MeSH Terms
Animals Cell Line DNA, Recombinant DNA, Viral/analysis Dexamethasone/pharmacology Gene Expression Regulation Genes Genes, Viral Genetic Markers Genetic Vectors Growth Hormone/biosynthesis,genetics Hypoxanthine Phosphoribosyltransferase/genetics Mice Operon Phenotype RNA, Viral/genetics Rats Retroviridae/genetics Transcription, Genetic Transfection Triiodothyronine/pharmacology
Chemicals
DNA, Recombinant DNA, Viral Genetic Markers RNA, Viral Triiodothyronine Dexamethasone Growth Hormone Hypoxanthine Phosphoribosyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Miller A D
Ong E S
Rosenfeld M G
Verma I M
Evans R M
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.6089340
Published
1984-09-07
Pages
993-8
Language
English
Region
United States
NLM ID
0404511
Subset
IM
External Links
PubMed source DOI: 10.1126/science.6089340 View journal details
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