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PMID: 6090438 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inverted repeat sequences flank a Bacillus thuringiensis crystal protein gene.

Journal of bacteriology ·Vol. 160 ·No. 1 ·1984-10-00 ·Pages 95-102

Kronstad JW, Whiteley HR

Abstract

Two sets of inverted repeat DNA sequences, IR2150 and IR1750, were discovered flanking the crystal protein gene on the 75-kilobase plasmid of Bacillus thuringiensis subsp. kurstaki HD73. A restriction map of ca. 40 kilobases around the crystal protein gene was constructed, and the positions of the copies of IR2150 and IR1750 were determined. Three copies of IR2150 were found flanking the crystal protein gene in an inverted orientation, and one partial and three intact copies of IR1750 were found in both inverted and direct orientations around the gene. Hybridization experiments with fragments from within IR2150 and IR1750 demonstrated the presence of multiple copies of these sequences on the chromosome of B. thuringiensis subsp. kurstaki HD73 and also revealed a strong correlation between the presence of these sequences and the presence of the crystal protein gene on plasmids from 14 strains of B. thuringiensis.

MeSH Terms
Bacillus thuringiensis/genetics Bacillus thuringiensis Toxins Bacterial Proteins/genetics Bacterial Toxins Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/isolation & purification Endotoxins Genes Genes, Bacterial Hemolysin Proteins Nucleic Acid Hybridization Plasmids
Chemicals
Bacillus thuringiensis Toxins Bacterial Proteins Bacterial Toxins DNA, Bacterial Endotoxins Hemolysin Proteins insecticidal crystal protein, Bacillus Thuringiensis DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kronstad J W
Whiteley H R
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.1.95-102.1984
Published
1984-10-00
Pages
95-102
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214686
Subset
IM
Grants
NIGMS NIH HHS · GM-07270-08 · United States
NIGMS NIH HHS · GM-20784 · United States
NIGMS NIH HHS · GM-26100 · United States
External Links
PubMed source DOI: 10.1128/jb.160.1.95-102.1984 PMC Full Text View journal details
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