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PMID: 6091111 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TK.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 18 ·1984-09-00 ·Pages 5821-5

Sclafani RA, Fangman WL

Abstract

The herpes simplex virus type 1 thymidine kinase gene TK complements the defect in five temperature-sensitive mutants and in vitro constructed insertion and deletion mutants of the CDC8 gene of Saccharomyces cerevisiae. The herpes thymidine kinase enzyme acts as both a thymidine kinase and a thymidylate kinase (dTMP kinase). The latter activity is responsible for the cdc8 complementation since all thermosensitive cdc8 mutants are deficient in dTMP kinase activity at all temperatures. However, an intragenic revertant, cdc8-320, which was selected by demanding mitotic growth at the restrictive temperature, exhibits thermolabile dTMP kinase activity. We conclude that CDC8 is the structural gene for dTMP kinase, which catalyzes an essential step in DNA precursor biosynthesis. Previously, it has been shown that the DNA replication defect of cdc8 mutants could not be bypassed by the addition of deoxyribonucleoside triphosphates to permeabilized cells. This apparent discrepancy can be explained by hypothesizing a multiprotein yeast DNA replication complex containing the CDC8 protein.

MeSH Terms
Base Sequence DNA Restriction Enzymes Escherichia coli/enzymology,genetics Genes Genes, Fungal Genes, Viral Genetic Complementation Test Mutation Nucleoside-Phosphate Kinase/genetics Phosphotransferases/genetics Plasmids Saccharomyces cerevisiae/enzymology,genetics Simplexvirus/enzymology,genetics Temperature Thymidine Kinase/genetics
Chemicals
Phosphotransferases Thymidine Kinase Nucleoside-Phosphate Kinase dTMP kinase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sclafani R A
Fangman W L
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.18.5821
Published
1984-09-00
Pages
5821-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391803
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.18.5821 PMC Full Text View journal details
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