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PMID: 6091124 Published · ppublish English Journal Article

Synthesis of a gene for human growth hormone and its expression in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 5956-60

Ikehara M, Ohtsuka E, Tokunaga T, Taniyama Y, Iwai S, Kitano K, Miyamoto S, Ohgi T, Sakuragawa Y, Fujiyama K

Abstract

A gene coding for human growth hormone, which consists of 192 amino acids, was chemically synthesized. The synthesis entailed ligating 78 deoxyribooligonucleotides, which had been synthesized on polymer supports by the phosphotriester method with frequently occurring amino acid codons of Escherichia coli. The chemically synthesized gene was inserted into an E. coli plasmid downstream from the E. coli trp promoter, with a modified ribosome-binding region carried on pBR322. E. coli cells transformed with this recombinant plasmid synthesized 2.9 X 10(6) molecules per cell of human growth hormone upon induction. The induced polypeptide was identical with natural human growth hormone in size and in immunological properties, as well as in biological activity as examined by the tibial test with hypophysectomized rats.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics,ultrastructure Genes Genes, Synthetic Growth Hormone/genetics Humans Indicators and Reagents Microscopy, Electron Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Operon Protein Biosynthesis Transcription, Genetic
Chemicals
Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Growth Hormone DNA Restriction Enzymes
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ikehara M
Ohtsuka E
Tokunaga T
Taniyama Y
Iwai S
Kitano K
Miyamoto S
Ohgi T
Sakuragawa Y
Fujiyama K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.19.5956
Published
1984-10-00
Pages
5956-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391837
Subset
IM
Databases
GENBANK
K02382
External Links
PubMed source DOI: 10.1073/pnas.81.19.5956 PMC Full Text View journal details
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