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PMID: 6091127 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Intermediate filament cDNAs from BHK-21 cells: demonstration of distinct genes for desmin and vimentin in all vertebrate classes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 5970-4

Quax W, van den Heuvel R, Egberts WV, Quax-Jeuken Y, Bloemendal H

Abstract

Recombinant cDNA plasmids for the intermediate filament proteins desmin and vimentin were constructed from baby hamster kidney (BHK-21) mRNA. Analysis of four desmin clones gave a sequence of 1574 nucleotides, which is 75% of the total mRNA length. The derived amino acid sequence for hamster desmin shows 92% overall homology with chicken desmin; the homology with hamster vimentin is highest in the alpha-helical middle part (74%). The 3'-noncoding region of desmin mRNA is found to be 677 nucleotides long. With the aid of 5'- and 3'-specific probes, it has been established that there is a single gene for desmin in the hamster genome. This gene expresses a single mRNA species of 2.2 kilobases. Hybridization experiments of a number of DNAs with desmin and vimentin probes show that there are distinct restriction enzyme fragments carrying vimentin and desmin sequences in the genome of representatives of all vertebrate classes.

MeSH Terms
Amino Acid Sequence Animals Cell Line Chickens Cricetinae DNA/metabolism DNA Restriction Enzymes Desmin/genetics Glial Fibrillary Acidic Protein/genetics Kidney Plasmids Protein Conformation RNA, Messenger/genetics Species Specificity Vimentin/genetics
Chemicals
Desmin Glial Fibrillary Acidic Protein RNA, Messenger Vimentin DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Quax W
van den Heuvel R
Egberts W V
Quax-Jeuken Y
Bloemendal H
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.19.5970
Published
1984-10-00
Pages
5970-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391840
Subset
IM
Databases
GENBANK
K02407
External Links
PubMed source DOI: 10.1073/pnas.81.19.5970 PMC Full Text View journal details
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