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PMID: 6092054 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 45-kb DNA domain with two divergently orientated genes is the unit of organisation of the murine major urinary protein genes.

The EMBO journal ·Vol. 3 ·No. 9 ·1984-09-00 ·Pages 2055-64

Clark AJ, Hickman J, Bishop J

Abstract

The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be distinguished by nucleic acid hybridisation. By screening a Charon 4A library of mouse DNA with probes from the 5'-flanking region of a MUP gene, we have isolated clones that contain both a Group 1 and a Group 2 gene, orientated in a divergent fashion, with 15 kb of DNA between the 5' ends of the genes. We show that this pairwise arrangement is the predominant organisation of MUP genes in the BALB/c genome. We argue that the head-to-head gene pair is the unit both of DNA organisation and of evolution. Taking into account the genes themselves, the intervening 15 kb and the homologous 3'-flanking regions, this unit is approximately 45 kb long. We also show that some MUP genes may be linked in a tail-to-tail fashion with 26-28 kb between the 3' ends of two genes. This suggests that the minimum distance between successive 45-kb units is approximately 7 kb.

MeSH Terms
Animals Base Composition Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes Genetic Linkage Mice Mice, Inbred BALB C Nucleic Acid Hybridization Plasmids Proteins/genetics RNA, Messenger/genetics
Chemicals
Proteins RNA, Messenger major urinary proteins DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clark A J
Hickman J
Bishop J
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-09-00
Pages
2055-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557643
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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