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PMID: 6092133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A gene fusion that localises the penicillin-binding domain of penicillin-binding protein 3 of Escherichia coli.

FEBS letters ·Vol. 176 ·No. 1 ·1984-10-15 ·Pages 179-84

Hedge PJ, Spratt BG

Abstract

A gene fusion that links the COOH-terminal 349 amino acids of penicillin-binding protein 3 (60 kDa) of E. coli to the NH2-terminus of beta-galactosidase has been constructed. The fusion protein (38.5 kDa) retains the ability to bind benzylpenicillin with high affinity, establishing that the penicillin-binding domain (and presumably the penicillin-sensitive transpeptidase activity) of this high molecular mass penicillin-binding protein is located on a COOH-terminal functional domain.

MeSH Terms

Bacterial Proteins/genetics,metabolism Carboxypeptidases/genetics Carrier Proteins/genetics,metabolism DNA Restriction Enzymes DNA, Bacterial/metabolism DNA, Recombinant/metabolism Escherichia coli/genetics Escherichia coli Proteins Hexosyltransferases Muramoylpentapeptide Carboxypeptidase/genetics,metabolism Penicillin G/metabolism Penicillin-Binding Proteins Penicillins/metabolism Peptide Fragments/genetics,metabolism Peptidoglycan Glycosyltransferase Peptidyl Transferases beta-Galactosidase/genetics

Chemicals

Bacterial Proteins Carrier Proteins DNA, Bacterial DNA, Recombinant Escherichia coli Proteins FtsI protein, E coli Penicillin-Binding Proteins Penicillins Peptide Fragments Peptidyl Transferases Hexosyltransferases Peptidoglycan Glycosyltransferase DNA Restriction Enzymes beta-Galactosidase Carboxypeptidases Muramoylpentapeptide Carboxypeptidase Penicillin G

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hedge P J

Spratt B G

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(84)80936-9

Published

1984-10-15

Pages

179-84

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(84)80936-9 View journal details

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A gene fusion that localises the penicillin-binding domain of penicillin-binding protein 3 of Escherichia coli.

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