Home LiteratureArticle Details
PMID: 6092874 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci.

Molecular & general genetics : MGG ·Vol. 196 ·No. 2 ·1984-00-00 ·Pages 367-72

Grogan DW, Cronan JE

Abstract

The phenotypically silent cyclopropane fatty acid synthesis (cfa) gene of Escherichia coli K-12 has been located on the genetic linkage map. This was accomplished by integrating (via homologous recombination) the selectable marker of a recombinant plasmid into the host chromosome near the cfa locus. This integration allowed the subsequent isolation of a cfa-linked transposon Tn10 insertion. Genetic mapping of the Tn10 insertion, using conventional techniques, placed the cfa locus at min 36.5 on the linkage map in the vicinity of several other non-selectable markers. We ordered cfa and these other loci by three-factor transductional analyses. Selection for excision of the Tn10 element resulted in several types of mutants which harbor mutations of cfa and of neighboring genes, presumably as a consequence of Tn10-catalyzed chromosomal rearrangements.

MeSH Terms
Bacterial Proteins/genetics Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Genes, Dominant Genetic Linkage Methyltransferases/genetics
Chemicals
Bacterial Proteins DNA Transposable Elements Methyltransferases cyclopropane synthetase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grogan D W
Cronan J E
References (22)
22 references, click to expand
  1. Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.
    J Bacteriol. 1981 Feb;145(2):914-9 PMID: 6257656
  2. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies.
    J Bacteriol. 1969 Jul;99(1):238-41 PMID: 4895844
  3. Selection and properties of Escherichia coli mutants defective in the synthesis of cyclopropane fatty acids.
    J Bacteriol. 1976 Feb;125(2):518-23 PMID: 1107324
  4. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  5. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  6. Cloning and mapping of the manganese superoxide dismutase gene (sodA) of Escherichia coli K-12.
    J Bacteriol. 1983 Sep;155(3):1078-87 PMID: 6309739
  7. Two genetic loci for resistance to kasugamycin in Escherichia coli.
    J Bacteriol. 1973 Feb;113(2):704-10 PMID: 4570603
  8. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10.
    J Mol Biol. 1979 Jan 5;127(1):89-115 PMID: 370414
  9. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein.
    J Bacteriol. 1978 Mar;133(3):1419-26 PMID: 417067
  10. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.
    J Bacteriol. 1972 Mar;109(3):971-8 PMID: 4551758
  11. Use of lambda phasmids for deletion mapping of non-selectable markers cloned in plasmids.
    Gene. 1983 Apr;22(1):75-83 PMID: 6305773
  12. Mapping nonselectable genes of Escherichia coli by using transposon Tn10: location of a gene affecting pyruvate oxidase.
    J Bacteriol. 1982 Sep;151(3):1279-89 PMID: 6286595
  13. Simplified method for interruption of conjugation in Escherichia coli.
    J Bacteriol. 1976 Apr;126(1):559-60 PMID: 770441
  14. Isolation of the Escherichia coli iron superoxide dismutase gene: evidence that intracellular superoxide concentration does not regulate oxygen-dependent synthesis of the manganese superoxide dismutase.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4970-3 PMID: 6087358
  15. Genetic characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein.
    J Bacteriol. 1977 Sep;131(3):759-64 PMID: 330496
  16. Identification of a novel genetic element in Escherichia coli K-12.
    J Bacteriol. 1980 Oct;144(1):312-21 PMID: 6998954
  17. Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli.
    J Bacteriol. 1974 Mar;117(3):1082-92 PMID: 4591943
  18. Selection for loss of tetracycline resistance by Escherichia coli.
    J Bacteriol. 1981 Feb;145(2):1110-1 PMID: 7007341
  19. On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1417-20 PMID: 323852
  20. Cyclopropane fatty acid synthase of Escherichia coli. Stabilization, purification, and interaction with phospholipid vesicles.
    Biochemistry. 1979 Jul 24;18(15):3292-300 PMID: 380648
  21. Restriction enzyme cleavage sites surrounding the structural gene for the lipoprotein of the Escherichia coli outer membrane.
    J Bacteriol. 1979 Jun;138(3):715-20 PMID: 378933
  22. Cloning and manipulation of the Escherichia coli cyclopropane fatty acid synthase gene: physiological aspects of enzyme overproduction.
    J Bacteriol. 1984 Apr;158(1):286-95 PMID: 6325391
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328074
Published
1984-00-00
Pages
367-72
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM26156 · United States
External Links
PubMed source DOI: 10.1007/BF00328074
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2