Short-term, high-efficiency expression of transfected DNA.
We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.
12 references, click to expand
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Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.J Natl Cancer Inst. 1968 Aug;41(2):351-7 PMID: 4299537
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A new technique for the assay of infectivity of human adenovirus 5 DNA.Virology. 1973 Apr;52(2):456-67 PMID: 4705382
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Co-transfer of human X-linked markers into murine somatic cells via isolated metaphase chromosomes.Proc Natl Acad Sci U S A. 1978 Jul;75(7):3346-50 PMID: 277934
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A better cell line for making hybridomas secreting specific antibodies.Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
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Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.J Virol. 1979 Aug;31(2):360-9 PMID: 225559
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Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA.Somatic Cell Genet. 1980 May;6(3):333-47 PMID: 6931407
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SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.Gene. 1981 Mar;13(2):197-202 PMID: 6263759
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Mitogenic effect of alkaline pH on quiescent, serum-starved cells.Proc Natl Acad Sci U S A. 1981 Jul;78(7):4334-8 PMID: 6945587
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Efficient DNA transfection and rapid assay for thymidine kinase activity and viral antigenic determinants.Somatic Cell Genet. 1981 Mar;7(2):161-70 PMID: 6170119
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Efficient infection of monkey cells with DNA of simian virus 40.Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8 PMID: 6278479
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The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81 PMID: 6294651
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Expression and stabilization of microinjected plasmids containing the herpes simplex virus thymidine kinase gene and polyoma virus DNA in mouse cells.Mol Cell Biol. 1983 Apr;3(4):511-22 PMID: 6304496
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