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PMID: 6094182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alternative splicing in the 5' moiety of the H-2Kd gene transcript.

The EMBO journal ·Vol. 3 ·No. 10 ·1984-10-00 ·Pages 2383-6

Transy C, Lalanne JL, Kourilsky P

Abstract

The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They correspond to two previously characterized cDNA clones isolated from DBA/2 mouse liver RNA, leading to the conclusion that the H-2Kd gene gives rise to two distinct transcripts through an alternate use of splicing sites. The non-canonical RNA potentially encodes a so far undescribed H-2Kd-like molecule. It is present in all tissues tested (liver, spleen, thymus, kidney) albeit in lower amounts (approximately 10-fold less) than the canonical RNA coding for H-2Kd.

MeSH Terms
Animals DNA/analysis Endonucleases/metabolism H-2 Antigens/genetics Liver/analysis Mice Mice, Inbred BALB C Mice, Inbred DBA RNA Splicing Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
H-2 Antigens H-2K(K) antigen DNA Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Transy C
Lalanne J L
Kourilsky P
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-10-00
Pages
2383-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557697
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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