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PMID: 6094483 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping and spacer identification of rRNA operons of Salmonella typhimurium.

Journal of bacteriology ·Vol. 160 ·No. 2 ·1984-11-00 ·Pages 682-6

Lehner AF, Harvey S, Hill CW

Abstract

The rRNA operons of Salmonella typhimurium have been characterized with respect to their map position, orientation, and type of tRNA spacer. One of the seven rrn operons was found to be linked to pheA and another was found to be linked to aroE. This information, together with published information about the other five rrn operons, shows that S. typhimurium and Escherichia coli are essentially identical in terms of the number, the map position, and the orientation of all seven operons. S. typhimurium and E. coli were also similar in that four of the rrn spacer regions code for tRNAGlu2 and three code for tRNAAla1B. However, the two species differed in that rrnD coded for tRNAGlu2 and rrnB coded for tRNAAla1B in S. typhimurium. This is the opposite of the arrangement in E. coli. We have tabulated the coordinates of the BamHI and PstI sites flanking six of the S. typhimurium rrn genes and present revisions for the coordinates of some of the E. coli sites.

MeSH Terms
Base Sequence Chromosome Mapping DNA Restriction Enzymes Escherichia coli/genetics Nucleic Acid Hybridization Operon RNA, Ribosomal/genetics RNA, Transfer/genetics Salmonella typhimurium/genetics Species Specificity
Chemicals
RNA, Ribosomal RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lehner A F
Harvey S
Hill C W
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.2.682-686.1984
Published
1984-11-00
Pages
682-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214789
Subset
IM
Grants
NCI NIH HHS · 1 P30 CA18450 · United States
NIGMS NIH HHS · GM16329 · United States
External Links
PubMed source DOI: 10.1128/jb.160.2.682-686.1984 PMC Full Text View journal details
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