Home LiteratureArticle Details
PMID: 6094496 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Level of rRNA, not tRNA, synthesis controls transcription of rRNA and tRNA operons in Escherichia coli.

Journal of bacteriology ·Vol. 160 ·No. 3 ·1984-12-00 ·Pages 1022-6

Gourse RL, Nomura M

Abstract

We have recently proposed a model for the negative feedback control of rRNA and tRNA synthesis in Escherichia coli by products of rRNA operons or their derivatives (e.g., nontranslating ribosomes) (S. Jinks-Robertson, R.L. Gourse, and M. Nomura, Cell 33:865-876, 1983). In this paper, we examined the following questions. (i) Are the spacer tRNAs carried within rRNA operons the products responsible for the regulation of rRNA and tRNA transcription? (ii) Are tRNAs capable of regulating their own syntheses? We measured tRNA accumulations in cells containing plasmids with intact or defective rRNA operons or with tRNA operons. From the results obtained, we conclude that neither the tRNAs encoded within rRNA operons nor the tRNAs encoded in non-rRNA operons are capable of controlling rRNA or tRNA transcription. Therefore, the products responsible for the initial step leading to rRNA and tRNA regulation are rRNAs (or their derivatives).

MeSH Terms
Aspartate-tRNA Ligase DNA Restriction Enzymes Escherichia coli/genetics Operon Plasmids RNA, Ribosomal/biosynthesis,genetics RNA, Transfer/biosynthesis,genetics RNA, Transfer, Amino Acyl/genetics Transcription, Genetic
Chemicals
RNA, Ribosomal RNA, Transfer, Amino Acyl RNA, Transfer DNA Restriction Enzymes Aspartate-tRNA Ligase asparaginyl-tRNA synthetase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gourse R L
Nomura M
References (19)
19 references, click to expand
  1. Glycine transfer RNA of Escherichia coli. I. Structural genes for two glycine tRNA species.
    J Mol Biol. 1970 Sep 28;52(3):557-69 PMID: 4923751
  2. Relationships among deoxyribonucleic acid, ribonucleic acid, and specific transfer ribonucleic acids in Escherichia coli 15T - at various growth rates.
    J Bacteriol. 1973 Jul;115(1):177-87 PMID: 4577741
  3. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  4. Chloramphenicol-induced changes in the synthesis of ribosomal, transfer, and messenger ribonucleic acids in Escherichia coli B/r.
    J Bacteriol. 1977 Jun;130(3):1098-108 PMID: 324974
  5. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli.
    Cell. 1977 Aug;11(4):779-93 PMID: 329999
  6. Gross map location of Escherichia coli transfer RNA genes.
    J Mol Biol. 1977 Dec 5;117(2):419-46 PMID: 342702
  7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  8. Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome.
    J Bacteriol. 1979 Jan;137(1):584-94 PMID: 368032
  9. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
  10. Sequence of the distal tRNA1Asp gene and the transcription termination signal in the Escherichia coli ribosomal RNA operon rrnF(or G).
    Nucleic Acids Res. 1980 Sep 11;8(17):3809-27 PMID: 6255418
  11. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes.
    J Mol Biol. 1981 Feb 15;146(1):1-21 PMID: 6167728
  12. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  13. Dual function transcripts specifying tRNA and mRNA.
    Nature. 1981 Dec 3;294(5840):422-7 PMID: 7312036
  14. Threonine tRNAs and their genes in Escherichia coli.
    Mol Gen Genet. 1982;187(1):132-7 PMID: 6761541
  15. Site-directed mutagenesis of ribosomal RNA. Construction and characterization of deletion mutants.
    J Mol Biol. 1982 Aug 15;159(3):397-416 PMID: 6187922
  16. Identification of clones carrying an E. coli tRNAPhe gene by suppression of phenylalanyl-tRNA synthetase thermosensitive mutants.
    Nucleic Acids Res. 1983 Feb 11;11(3):727-36 PMID: 6300764
  17. Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe.
    Nucleic Acids Res. 1983 Jul 11;11(13):4379-89 PMID: 6306588
  18. Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons.
    Cell. 1983 Jul;33(3):865-76 PMID: 6191870
  19. Regulation of the synthesis of ribosomes and ribosomal components.
    Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.3.1022-1026.1984
Published
1984-12-00
Pages
1022-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215812
Subset
IM
Grants
NIGMS NIH HHS · GM-20427 · United States
External Links
PubMed source DOI: 10.1128/jb.160.3.1022-1026.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2