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PMID: 6096813 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).

Nucleic acids research ·Vol. 12 ·No. 23 ·1984-12-11 ·Pages 8939-49

Ono A, Sato M, Ohtani Y, Ueda T

Abstract

Deoxydecanucleotides having a recognition sequence of Bgl II and Sau 3AI, and their 7-deazaadenine analogs were synthesized. The decanucleotides containing 7-deazaadenine in place of adenine were partially resistant to the hydrolysis by Sau 3AI and strongly resistant to that by Bgl II. A new hypothesis on the mode of recognition and cleavage of specific nucleotide sequences by Bgl II, recognizing one strand and cleaving the other strand, is presented.

MeSH Terms

Adenine/analogs & derivatives Bacterial Proteins Base Sequence DNA Restriction Enzymes/metabolism Deoxyribonucleases, Type II Site-Specific Indicators and Reagents Kinetics Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Phosphorus Radioisotopes Structure-Activity Relationship Substrate Specificity

Chemicals

Bacterial Proteins Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Phosphorus Radioisotopes 7-deazaadenine DNA Restriction Enzymes BglII endonuclease Deoxyribonucleases, Type II Site-Specific GATC-specific type II deoxyribonucleases Adenine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ono A

Sato M

Ohtani Y

Ueda T

References (8)

8 references, click to expand

DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.
Nucleic Acids Res. 1974 Mar;1(3):331-53 PMID: 10793670
Sequence-specific recognition of double helical nucleic acids by proteins.
Proc Natl Acad Sci U S A. 1976 Mar;73(3):804-8 PMID: 1062791
A restriction endonuclease from Staphylococcus aureus.
Nucleic Acids Res. 1976 Nov;3(11):3193-202 PMID: 1005115
Effect of nucleotide analogs on the cleavage of DNA by the restriction enzymes AluI, DdeI, HinfI, RsaI, and TaqI.
J Biol Chem. 1983 Dec 25;258(24):15206-13 PMID: 6317689
Purification and properties of the Bg1I and II endonucleases.
Methods Enzymol. 1980;65(1):132-8 PMID: 6246333
Structures and mechanisms of DNA restriction and modification enzymes.
Q Rev Biophys. 1979 Aug;12(3):315-69 PMID: 232555
Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.
Nucleic Acids Res. 1980 Nov 25;8(22):5507-17 PMID: 7465420
Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii.
J Bacteriol. 1978 Apr;134(1):338-44 PMID: 649568

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.23.8939

Published

1984-12-11

Pages

8939-49

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320430

Subset

External Links

PubMed source DOI: 10.1093/nar/12.23.8939 PMC Full Text View journal details

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Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).

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