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PMID: 6105150 Published · ppublish English Journal Article

Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b.

Journal of bacteriology ·Vol. 143 ·No. 1 ·1980-07-00 ·Pages 411-5

Premakumar R, Sorger GJ, Gooden D

Abstract

The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine could repress nitrate reductase. In a mutant of Neurospora, gln-1b, which requires glutamine for growth, only glutamine could repress nitrate reductase. These results suggest a direct role for glutamine as corepressor of nitrate reductase in Neurospora.

MeSH Terms
Enzyme Repression/drug effects Glutamate-Ammonia Ligase/antagonists & inhibitors Glutamine/pharmacology Methionine Sulfoximine/pharmacology Neurospora/enzymology Nitrate Reductases/biosynthesis Quaternary Ammonium Compounds/pharmacology
Chemicals
Quaternary Ammonium Compounds Glutamine Methionine Sulfoximine Nitrate Reductases Glutamate-Ammonia Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Premakumar R
Sorger G J
Gooden D
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.1.411-415.1980
Published
1980-07-00
Pages
411-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294258
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.143.1.411-415.1980 PMC Full Text View journal details
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