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PMID: 6111299 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Subcellular localization of long-chain alcohol dehydrogenase and aldehyde dehydrogenase in n-alkane-grown Candida tropicalis.

Archives of microbiology ·Vol. 128 ·No. 2 ·1980-12-00 ·Pages 145-51

Yamada T, Nawa H, Kawamoto S, Tanaka A, Fukui S

Abstract

Long-chain alcohol dehydrogenase and long-chain aldehyde dehydrogenase were induced in the cells of Candida tropicalis grown on n-alkanes. Subcellular localization of these dehydrogenases, together with that of acyl-CoA synthetase and glycerol-3-phosphate acyltransferase, was studied in terms of the metabolism of fatty acids derived from n-alkane substrates. Both long-chain alcohol and aldehyde dehydrogenases distributed in the fractions of microsomes, mitochondria and peroxisomes obtained from the alkane-grown cells of C. tropicalis. Acyl-CoA synthetase was also located in these three fractions. Glycerol-3-phosphate acyltransferase was found in microsomes and mitochondria, in contrast to fatty acid beta-oxidation system localized exclusively in peroxisomes. Similar results of the enzyme localization were also obtained with C. lipolytica grown on n-alkanes. These results suggest strongly that microsomal and mitochondrial dehydrogenases provide long-chain fatty acids to be utilized for lipid synthesis, whereas those in peroxisomes supply fatty acids to be degraded via beta-oxidation to yield energy and cell constituents.

MeSH Terms
Acetate-CoA Ligase/metabolism Alcohol Oxidoreductases/metabolism Aldehyde Oxidoreductases/metabolism Alkanes/metabolism Candida/enzymology,metabolism Glycerolphosphate Dehydrogenase/metabolism Microbodies/enzymology Microsomes/enzymology Mitochondria/enzymology
Chemicals
Alkanes Alcohol Oxidoreductases Glycerolphosphate Dehydrogenase Aldehyde Oxidoreductases Acetate-CoA Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yamada T
Nawa H
Kawamoto S
Tanaka A
Fukui S
References (19)
19 references, click to expand
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00406151
Published
1980-12-00
Pages
145-51
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00406151 View journal details
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