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PMID: 6111627 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A simple perfusion chamber for studying neurotransmitter release from cells maintained in monolayer culture.

Journal of neuroscience methods ·Vol. 3 ·No. 3 ·1981-02-00 ·Pages 255-9

Pearce BR, Currie DN, Dutton GR, Hussey RE, Beale R, Pigott R

Abstract

A perfusion chamber is described for studying the efflux of putative neurotransmitters from CNS cells maintained in monolayer culture. We have used this apparatus to investigate the efflux of newly accumulated [3H]GABA from cell cultures of the early postnatal rat cerebellum.

MeSH Terms
Animals Cells, Cultured Cerebellum/metabolism Neurotransmitter Agents/metabolism Perfusion/instrumentation,methods Rats gamma-Aminobutyric Acid/metabolism
Chemicals
Neurotransmitter Agents gamma-Aminobutyric Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pearce B R
Currie D N
Dutton G R
Hussey R E
Beale R
Pigott R
Article Info
Journal
Journal of neuroscience methods
Abbr.
J Neurosci Methods
ISSN
0165-0270
DOI
10.1016/0165-0270(81)90061-3
Published
1981-02-00
Pages
255-9
Language
English
Region
Netherlands
NLM ID
7905558
Subset
IM
External Links
PubMed source DOI: 10.1016/0165-0270(81)90061-3 View journal details
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