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PMID: 6116675 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.

Infection and immunity ·Vol. 33 ·No. 3 ·1981-09-00 ·Pages 933-8

Hull RA, Gill RE, Hsu P, Minshew BH, Falkow S

Abstract

Isolates of Escherichia coli from human urinary tract infections frequently express adherence properties found less often among normal intestinal isolates. These properties include adherence to human uroepithelial cells and primary monkey kidney cells, as well as D-mannose-resistant hemagglutination of human erythrocytes, and they are mediated by a pilus type different from type 1. The genes encoding this pilus type (pyelonephritis-associated pili, pap) and those encoding type 1 pili have been cloned from a urinary tract infection isolate of E. coli and transferred to an E. coli K-12 derivative. The recombinant plasmids were found to express functional pili and to endow the new host with all of the adherence properties of the urinary tract infection isolate. Both pilus types were found to be genetically distinct, and unlike the adherence genes from bovine, porcine, and human diarrheal isolates, both were found to be chromosomally encoded.

MeSH Terms
Adhesiveness DNA Restriction Enzymes DNA, Recombinant Escherichia coli/genetics,pathogenicity,ultrastructure Escherichia coli Infections/microbiology Fimbriae, Bacterial Genes, Bacterial Hemagglutination Mannose/pharmacology Urinary Tract Infections/microbiology
Chemicals
DNA, Recombinant DNA Restriction Enzymes Mannose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hull R A
Gill R E
Hsu P
Minshew B H
Falkow S
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19 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.33.3.933-938.1981
Published
1981-09-00
Pages
933-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350799
Subset
IM
Grants
NIAID NIH HHS · AI00290 · United States
NIAID NIH HHS · AI10885 · United States
NIAID NIH HHS · AI14740 · United States
External Links
PubMed source DOI: 10.1128/iai.33.3.933-938.1981 PMC Full Text View journal details
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