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PMID: 6120174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.

The Journal of cell biology ·Vol. 92 ·No. 1 ·1982-01-00 ·Pages 108-12

Lin JJ, Queally SA

Abstract

We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double-staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin.

MeSH Terms
Animals Antibodies, Monoclonal Cells, Cultured Demecolcine/pharmacology Gerbillinae Golgi Apparatus/immunology Intracellular Membranes/immunology Membrane Proteins/immunology Molecular Weight Monensin Thiamine Pyrophosphatase/metabolism
Chemicals
Antibodies, Monoclonal Membrane Proteins Monensin Thiamine Pyrophosphatase Demecolcine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lin J J
Queally S A
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20 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.92.1.108
Published
1982-01-00
Pages
108-12
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112016
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.92.1.108 PMC Full Text View journal details
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