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PMID: 6123113 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of tyrosine aminotransferase mRNA by glucocorticoids and cAMP in fetal rat liver.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 2 ·1982-01-00 ·Pages 365-8

Ruiz-Bravo N, Ernest MJ

Abstract

Tyrosine aminotransferase (L-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5) enzyme and mRNA activity were not detectable in day 20 fetal rat liver. Precocious induction of catalytic activity by in utero injection of dibutyryl cAMP was a direct consequence of the de novo appearance of translatable tyrosine aminotransferase mRNA. In contrast, in utero injection of hydrocortisone acetate failed to elicit fetal liver enzyme activity. This failure was due to the inability of the steroid hormone to induce the appearance of tyrosine aminotransferase mRNA activity. In fetal rat liver explants, either compound was capable of stimulating the synthesis of adult levels of enzyme and mRNA. However, catalytic and mRNA activity comparable with that seen in vivo 24 hr after birth required the concerted action of both inducers.

MeSH Terms
Animals Bucladesine/pharmacology Culture Techniques Enzyme Induction/drug effects Gene Expression Regulation/drug effects Hydrocortisone/pharmacology Kinetics Liver/embryology,enzymology Protein Biosynthesis RNA, Messenger/biosynthesis Rats Transcription, Genetic/drug effects Tyrosine Transaminase/genetics
Chemicals
RNA, Messenger Bucladesine Tyrosine Transaminase Hydrocortisone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ruiz-Bravo N
Ernest M J
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.2.365
Published
1982-01-00
Pages
365-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345735
Subset
IM
Grants
NIADDK NIH HHS · AM 22030 · United States
NICHD NIH HHS · HD 07180 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.2.365 PMC Full Text View journal details
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