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PMID: 6123499 Published · ppublish English Comparative Study Journal Article

Nitrogen control in Pseudomonas aeruginosa: mutants affected in the synthesis of glutamine synthetase, urease, and NADP-dependent glutamate dehydrogenase.

Journal of bacteriology ·Vol. 151 ·No. 1 ·1982-07-00 ·Pages 22-8

Janssen DB, Habets WJ, Marugg JT, Van Der Drift C

Abstract

Mutants were isolated from Pseudomonas aeruginosa that were impaired in the utilization of a number of nitrogen sources. In contrast to the wild-type strain, these mutants appeared to be unable to derepress the formation of glutamine synthetase and urease under nitrogen-limited growth conditions, whereas NADP-dependent glutamate dehydrogenase became derepressed. This GlnR- phenotype appeared to be caused by a mutation located in the early region of the P. aeruginosa PAO chromosomal map, close to hisIV59. Partial suppression of the GlnR- phenotype due to a mutation located close to hisII4 was observed. These revertants were different from both the wild-type strain and the GlnR- mutant with respect to the regulation of the synthesis of glutamine synthetase, urease, and NADP-dependent glutamate dehydrogenase (GlnRc phenotype). Also the regulation of glutamine synthetase activity by adenylylation/deadenylylation was altered in the revertants. The results suggest the presence of a regulatory gene that plays a role in the regulation of enzyme formation in response to the availability of ammonia.

MeSH Terms
Chromosomes, Bacterial/physiology Genotype Glutamate Dehydrogenase/genetics Glutamate-Ammonia Ligase/genetics Mutation NADP Nitrogen/metabolism Pseudomonas aeruginosa/enzymology,genetics Species Specificity Urease/genetics
Chemicals
NADP Glutamate Dehydrogenase Urease Glutamate-Ammonia Ligase Nitrogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Janssen D B
Habets W J
Marugg J T
Van Der Drift C
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.1.22-28.1982
Published
1982-07-00
Pages
22-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220179
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.151.1.22-28.1982 PMC Full Text View journal details
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