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PMID: 6132382 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional changes of intermediate filaments in fibroblastic cells revealed by a monoclonal antibody.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 7 ·1983-04-00 ·Pages 1915-8

Dulbecco R, Allen R, Okada S, Bowman M

Abstract

We describe reversible changes of intermediate filaments of fibroblastic cells associated with changes in the functional state of the cells. The changes are revealed by comparing the immunofluorescence patterns given by a monoclonal antibody and a polyclonal serum, both recognizing vimentin. The state of the filaments depends on culture density; this effect cannot be attributed to the nutritional state of the cells, their growth rate, or substances released into the medium. It seems to depend mainly on the aggregation of filaments during strong cell movements. The possible significance of these findings for the functional role of intermediate filaments is discussed.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Surface/immunology Cell Adhesion Cell Movement Cells, Cultured Cross Reactions Cytoskeleton/ultrastructure Fibroblasts/ultrastructure Intermediate Filament Proteins/immunology Mice Thy-1 Antigens Vimentin Wound Healing
Chemicals
Antibodies, Monoclonal Antigens, Surface Intermediate Filament Proteins Thy-1 Antigens Vimentin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dulbecco R
Allen R
Okada S
Bowman M
References (25)
25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.7.1915
Published
1983-04-00
Pages
1915-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393721
Subset
IM
Grants
NCI NIH HHS · 1-R01CA21993 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.7.1915 PMC Full Text View journal details
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