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PMID: 6135974 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The E. coli uvrD gene product is DNA helicase II.

Molecular & general genetics : MGG ·Vol. 190 ·No. 2 ·1983-00-00 ·Pages 265-70

Hickson ID, Arthur HM, Bramhill D, Emmerson PT

Abstract

We have shown that the uvrD gene product, previously identified in maxicell extracts as a 73 kilodalton protein, copurifies with single stranded DNA-dependent ATPase and ATP-dependent DNA helicase activities. This protein is specifically precipitated from maxicell extracts by antibodies raised against DNA helicase II. In order to facilitate purification of the UvrD protein we have subcloned the uvrD gene into a plasmid vector in which its transcription is under the control of the phage lambda leftward promoter. Using cells harbouring this recombinant plasmid as a source of elevated levels of the UvrD protein we have purified this protein to homogeneity by a simple, rapid procedure. The purified protein has single stranded DNA-dependent ATPase activity and ATP-dependent DNA helicase activity, and both activities are specifically inactivated by antibodies raised against DNA helicase II. We conclude that DNA helicase II is the uvrD gene product.

MeSH Terms
Adenosine Triphosphatases/genetics,immunology,isolation & purification Antibodies/immunology Bacterial Proteins/genetics Cloning, Molecular DNA Helicases Escherichia coli/analysis,genetics Escherichia coli Proteins Genes, Bacterial Plasmids
Chemicals
Antibodies Bacterial Proteins Escherichia coli Proteins Adenosine Triphosphatases UvrD protein, E coli DNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hickson I D
Arthur H M
Bramhill D
Emmerson P T
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330649
Published
1983-00-00
Pages
265-70
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330649
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