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PMID: 6136038 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunocytochemical localization of gamma-glutamyltransferase in induced hyperplastic nodules of rat liver.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 15 ·1983-08-00 ·Pages 4742-6

Spater HW, Quintana N, Becker FF, Novikoff AB

Abstract

The immunocytochemical localization of gamma-glutamyltransferase [(5-glutamyl)-peptide:amino-acid 5-glutamyltransferase, EC 2.3.2.2; gamma-GluTase] was demonstrated in hyperplastic liver induced by the carcinogen 2-acetylaminofluorene (2-AAF). The method used a specific antiserum and protein A-horseradish peroxidase and permitted visualization of antigenic sites at both the light and electron microscopic levels. Electron microscopy revealed deposits of 3,3'-diaminobenzidine (DAB) reaction product in the plasma membranes of (i) hyperplastic cells, (ii) bile canaliculi, (iii) endothelial cell membranes, and (iv) lymphocytes. The so-called ATPase activity was localized in the plasma membrane in bile canaliculi and in endothelial cells; the hyperplastic cells show marked variability in the levels of this activity.

MeSH Terms
2-Acetylaminofluorene/toxicity Acyltransferases/metabolism Adenosine Triphosphatases/metabolism Animals Histocytochemistry Hyperplasia/enzymology Immunoenzyme Techniques Liver/drug effects,enzymology,pathology,ultrastructure Male Microscopy, Electron Rats Rats, Inbred Strains Transglutaminases
Chemicals
2-Acetylaminofluorene Acyltransferases Transglutaminases Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Spater H W
Quintana N
Becker F F
Novikoff A B
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.15.4742
Published
1983-08-00
Pages
4742-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384120
Subset
IM
Grants
NCI NIH HHS · CA06576 · United States
NCI NIH HHS · CA14923 · United States
NCI NIH HHS · CA20659 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.15.4742 PMC Full Text View journal details
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