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PMID: 6136965 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Biosynthesis of aromatic compounds: 13C NMR spectroscopy of whole Escherichia coli cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 19 ·1982-10-00 ·Pages 5828-32

Ogino T, Garner C, Markley JL, Herrmann KM

Abstract

13C and 31P NMR spectra of wild-type Escherichia coli showed resonances from metabolic intermediates of glycolysis and ATP formation but no detectable signals from aromatic amino acids. However, tyrosine biosynthesis from D-[l-13C]glucose was observed in cells harboring a feedback-resistant allele of aroF, the gene encoding tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase [7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), EC4.1.2.15], one of the isoenzymes that control carbon flow through the common aromatic biosynthetic pathway. A similar accumulation of tyrosine and phenylalanine is seen in cells carrying a multiple-copy plasmid that carries a wild-type aroF allele in addition to pheA and tyrA, the structural genes for controlling enzymes of the terminal pathways to phenylalanine and tyrosine biosynthesis. These in vivo measurements by a noninvasive probe suggest feedback inhibition as the quantitatively major mechanism controlling carbon flow in the common aromatic compound biosynthetic pathway. In strains accumulating aromatic amino acids, a transient accumulation of trehalose was detected, indicating that previously unknown changes in Escherichia coli metabolism accompany overproduction of aromatic compounds.

MeSH Terms
3-Deoxy-7-Phosphoheptulonate Synthase/genetics Aldehyde-Lyases/genetics Escherichia coli/genetics,metabolism Feedback Genes Genes, Bacterial Genotype Magnetic Resonance Spectroscopy Phenotype Species Specificity Tyrosine/biosynthesis
Chemicals
Tyrosine 3-Deoxy-7-Phosphoheptulonate Synthase Aldehyde-Lyases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ogino T
Garner C
Markley J L
Herrmann K M
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.19.5828
Published
1982-10-00
Pages
5828-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347003
Subset
IM
Grants
NIGMS NIH HHS · GM07211 · United States
NIGMS NIH HHS · GM19907 · United States
NCRR NIH HHS · RR-01077 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.19.5828 PMC Full Text View journal details
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