Home LiteratureArticle Details
PMID: 6139361 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides.

Journal of bacteriology ·Vol. 156 ·No. 3 ·1983-12-00 ·Pages 1001-5

Neale GA, Mitchell A, Finch LR

Abstract

Cell-free extracts of Mycoplasma mycoides subsp. mycoides were assayed for enzymes associated with the salvage synthesis of pyrimidine deoxyribonucleotides. They possessed kinases for deoxycytidine, (d)CMP, thymidine (deoxyuridine), dTMP, and nucleoside diphosphates; dCTPase and dUTPase; dCMP deaminase; thymidine (deoxyuridine) phosphorylase; and dUMP (dTMP) phosphatase. The existence of these enzymic activities together with ribonucleoside diphosphate reductase explains the capacity of cytidine to provide M. mycoides with deoxyribose for the synthesis of thymidine nucleotides from thymine.

MeSH Terms
DCMP Deaminase/metabolism Deoxycytidine Kinase/metabolism Mycoplasma mycoides/enzymology Nucleoside-Diphosphate Kinase/metabolism Nucleoside-Phosphate Kinase/metabolism Phosphoric Monoester Hydrolases/metabolism Pyrimidine Nucleotides/metabolism Pyrophosphatases/metabolism Thymidine Kinase/metabolism Thymidine Phosphorylase/metabolism
Chemicals
Pyrimidine Nucleotides Thymidine Phosphorylase Thymidine Kinase Deoxycytidine Kinase cytidylate kinase Nucleoside-Phosphate Kinase Nucleoside-Diphosphate Kinase dTMP kinase Phosphoric Monoester Hydrolases DCMP Deaminase Pyrophosphatases dCTP pyrophosphatase dUTP pyrophosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Neale G A
Mitchell A
Finch L R
References (18)
18 references, click to expand
  1. Enzymatic synthesis of deoxyribonucleotides. IX. Allosteric effects in the reduction of pyrimidine ribonucleotides by the ribonucleoside diphosphate reductase system of Escherichia coli.
    J Biol Chem. 1966 Jun 10;241(11):2533-9 PMID: 5330119
  2. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.
    Nature. 1965 Apr 3;206:93 PMID: 14334364
  3. A defined medium for Mycoplasma strain Y.
    J Gen Microbiol. 1969 Sep;58(1):39-47 PMID: 4243269
  4. On the regulation of a bacterial deoxycytidylate deaminase.
    J Biol Chem. 1971 Dec 25;246(24):7755-8 PMID: 5002683
  5. Regulation of enzymatic activity through subunit interaction. A possible example.
    J Biol Chem. 1972 Dec 25;247(24):8136-8 PMID: 4640940
  6. Deoxycytidine triphosphate deaminase of Salmonella typhimurium. Purification and characterization.
    J Biol Chem. 1975 Jan 25;250(2):609-16 PMID: 234434
  7. Striated fibers of the rho form of Mycoplasma: in vitro reassembly, composition, and structure.
    J Bacteriol. 1975 Jun;122(3):1216-29 PMID: 238941
  8. Pathways of nucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides.
    J Bacteriol. 1977 Jun;130(3):1047-54 PMID: 324972
  9. Deoxycytidylate deaminase from Bacillus subtilis. Purification, characterization, and physiological function.
    J Biol Chem. 1978 May 25;253(10):3536-42 PMID: 418064
  10. Enzymes of purine metabolism in Mycoplasma mycoides subsp. mycoides.
    J Bacteriol. 1978 Jun;134(3):706-12 PMID: 207675
  11. Enzymes of pyrimidine metabolism in Mycoplasma mycoides subsp. mycoides.
    J Bacteriol. 1979 Mar;137(3):1073-80 PMID: 220209
  12. Allosteric regulation of calf thymus ribonucleoside diphosphate reductase.
    Biochemistry. 1979 Jul 10;18(14):2948-52 PMID: 223624
  13. Phylogenetic analysis of the mycoplasmas.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):494-8 PMID: 6928642
  14. A nonlinear regression program for small computers.
    Anal Biochem. 1981 Jan 1;110(1):9-18 PMID: 7212273
  15. Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides.
    J Bacteriol. 1983 Apr;154(1):17-22 PMID: 6339469
  16. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  17. Nutrition and metabolism of Mycoplasma mycoides var. mycoides.
    Ann N Y Acad Sci. 1960 Jan 15;79:499-507 PMID: 14438252
  18. Enzymatic synthesis of deoxyribonucleotides. X. Reduction of purine ribonucleotides; allosteric behavior and substrate specificity of the enzyme system from Escherichia coli B.
    J Biol Chem. 1966 Jun 10;241(11):2540-9 PMID: 5330120
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.3.1001-1005.1983
Published
1983-12-00
Pages
1001-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217942
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.156.3.1001-1005.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2