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PMID: 6142055 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Enzyme-linked immunosorbent assay with a monoclonal antibody for detecting group A meningococcal antigens in cerebrospinal fluid.

Journal of clinical microbiology ·Vol. 19 ·No. 2 ·1984-02-00 ·Pages 230-4

Sugasawara RJ, Prato CM, Sippel JE

Abstract

Hybridomas were produced from spleen cells of BALB/c mice immunized with a membrane preparation from Neisseria meningitidis group A strain 4402 and S194/5.XXOBU.14 myeloma cells. The hybridomas were screened for secretion of antibodies suitable for an enzyme-linked immunosorbent assay (ELISA) diagnostic for group A meningococcal meningitis. One hybridoma antibody, 3G7, was directed against the pilus protein. This antibody bound to all six lipopolysaccharide and protein group A meningococcal serotyping strains, as well as to meningococcal strains from serogroups C, W135, and Y, but not to a strain of Escherichia coli, Haemophilus influenzae type b, or to two or more strains of Streptococcus pneumoniae, Neisseria gonorrhoeae, and Salmonella typhi. The ELISA used on antibody, antigen, antibody-conjugate sandwich. Rabbit anti-meningococcal serum was the coating antibody for the antibody sandwich, cerebrospinal fluids contained the bacterial antigens, and 3G7-alkaline phosphatase conjugate was the detecting antibody. The monoclonal antibody conjugate ELISA system was able to detect group A meningococcal antigens in 21 of 25 cerebrospinal fluid specimens that were positive in an immune rabbit serum conjugate ELISA; cerebrospinal fluid samples from patients with Haemophilus meningitis served as the controls. Counterimmunoelectrophoresis detected meningococcal antigens in 16 of the same 25 cerebrospinal fluid samples.

MeSH Terms
Antibodies, Monoclonal/immunology Antibody Specificity Antigens, Bacterial/cerebrospinal fluid Enzyme-Linked Immunosorbent Assay Fimbriae, Bacterial/immunology Humans Neisseria meningitidis/immunology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sugasawara R J
Prato C M
Sippel J E
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.19.2.230-234.1984
Published
1984-02-00
Pages
230-4
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC271027
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.19.2.230-234.1984 PMC Full Text View journal details
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