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PMID: 6143251 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of the insulin receptor in mediating the insulin-stimulated growth response in Reuber H-35 cells.

Molecular and cellular biochemistry ·Vol. 58 ·No. 1-2 ·1984-00-00 ·Pages 139-46

Koontz JW

Abstract

Insulin is able to stimulate a growth response in a variety of different cell types. However, the role of the insulin receptor in mediating this response is not clear. Indeed, it has been reported that the ability of insulin to stimulate a growth response is a result of its interaction with other growth factor receptors rather than the insulin receptor. We have previously reported that the H-35 hepatoma cell line responded to physiological concentrations of insulin as a growth factor and that the relative potency of proinsulin suggested that this response was mediated by the insulin receptor. In this report, two experimental approaches are used to demonstrate the involvement of the insulin receptor in mediating the growth response. Two different preparations of antibody to the insulin receptor are found to be capable of stimulating this response. In addition, the human insulin-like growth factors (IGF-I and II) show very low cross-reactivity with the insulin receptor and are significantly less potent than insulin in stimulating the growth response.

MeSH Terms
Animals Antigen-Antibody Reactions Cell Cycle/drug effects Cells, Cultured Enzyme Induction/drug effects Humans Insulin/pharmacology Liver Neoplasms, Experimental Peptides/pharmacology Receptor, Insulin/immunology,physiology Receptors, Cell Surface/physiology Receptors, Somatomedin Somatomedins/pharmacology Tyrosine Transaminase/metabolism
Chemicals
Insulin Peptides Receptors, Cell Surface Receptors, Somatomedin Somatomedins Tyrosine Transaminase Receptor, Insulin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Koontz J W
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31 references, click to expand
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00240613
Published
1984-00-00
Pages
139-46
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
Grants
PHS HHS · 7082 · United States
NIADDK NIH HHS · AM 30512 · United States
External Links
PubMed source DOI: 10.1007/BF00240613 View journal details
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