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PMID: 6149549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional activation of the rat liver tyrosine aminotransferase gene by cAMP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 21 ·1984-11-00 ·Pages 6637-41

Hashimoto S, Schmid W, Schütz G

Abstract

The enzyme tyrosine aminotransferase (Tyr-ATase; L-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5), which is synthesized in rat liver, is induced by glucocorticoids, insulin, and glucagon or its intracellular mediator cAMP. We have used cloned TyrATase genomic and cDNA sequences to study the mechanism of induction by cAMP. RNA blot analysis shows that cAMP causes a rapid 5-fold increase in TyrATase mRNA concentration in rat liver. Transcription in isolated rat liver nuclei was studied to determine the relative rate of transcription of the TyrATase gene after cAMP administration. We show that the accumulation of TyrATase mRNA after cAMP stimulation is a consequence of transcriptional activation of the TyrATase gene. Combined dexamethasone and cAMP treatment leads to higher TyrATase mRNA concentrations than each inducer alone, which implies that dexamethasone and cAMP act by distinct mechanisms.

MeSH Terms
Animals Bucladesine/pharmacology Cell Nucleus/enzymology Cyclic AMP/pharmacology DNA, Recombinant Dexamethasone/pharmacology Drug Interactions Kinetics Liver/enzymology Male Nucleic Acid Hybridization RNA, Messenger/metabolism Rats Rats, Inbred Strains Transcription, Genetic/drug effects Tyrosine Transaminase/genetics
Chemicals
DNA, Recombinant RNA, Messenger Bucladesine Dexamethasone Cyclic AMP Tyrosine Transaminase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hashimoto S
Schmid W
Schütz G
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.21.6637
Published
1984-11-00
Pages
6637-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391985
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.21.6637 PMC Full Text View journal details
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