Home LiteratureArticle Details
PMID: 6153212 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and partial characterization of the human homologue of Thy-1.

The Journal of experimental medicine ·Vol. 151 ·No. 2 ·1980-02-01 ·Pages 400-6

Ades EW, Zwerner RK, Acton RT, Balch CM

Abstract

A glycoprotein of 25,000 daltons was isolated from a human T lymphoblastoid cell line (MOLT-3). A monovalent antiserum raised against this antigen precipitated mouse Thy-1.1 antigen as well as a 25,000-dalton antigen from human blood T cells. The unabsorbed serum was also cytotoxic for both mouse and human T cells. By multiple criteria, this human T antigen appears to be homologous with mouse Thy-1.

MeSH Terms
Animals Antigens, Surface/isolation & purification Cell Differentiation Cell Line Cross Reactions Epitopes Glycoproteins/immunology Humans Mice Molecular Weight Species Specificity T-Lymphocytes/immunology
Chemicals
Antigens, Surface Epitopes Glycoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ades E W
Zwerner R K
Acton R T
Balch C M
References (21)
21 references, click to expand
  1. Mouse thymic iso-antigens.
    Nature. 1966 Jan 29;209(5022):521-3 PMID: 5919593
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. The immune system: a model for differentiation in higher organisms.
    Adv Immunol. 1971;14:291-351 PMID: 4943911
  4. Occurrence of a theta-like antigen in rats.
    J Exp Med. 1972 Nov 1;136(5):1054-62 PMID: 4117187
  5. Theta alloantigen on mouse and rat fibroblasts.
    Nat New Biol. 1973 Nov 21;246(151):76-8 PMID: 4357310
  6. Immunoglobulin classes in mammalian species identifed by cross-reactivity with antisera to human immunoglobulin.
    Immunochemistry. 1973 Dec;10(12):805-13 PMID: 4203355
  7. Precipitin cross-reactions between human and animal J-chains.
    Adv Exp Med Biol. 1974;45(0):251-5 PMID: 4213301
  8. Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes.
    Biochem J. 1975 Dec;151(3):685-97 PMID: 56177
  9. Purification of the Thy-1 molecule from rat brain.
    Biochem J. 1975 Dec;151(3):699-706 PMID: 56178
  10. Studies on the human T-lymphocyte population - IV. The isolation of T-lymphocyte antigens from peripheral lymphocytes.
    Immunochemistry. 1976 Feb;13(2):121-7 PMID: 57088
  11. Cross-reactive T-cell antigens among mammalian species.
    J Immunol. 1976 Aug;117(2):447-9 PMID: 59778
  12. Solubilization and molecular characterization of membrane-bound antigens shared by thymocytes and brain.
    Eur J Immunol. 1976 May;6(5):333-40 PMID: 62668
  13. Isolation and characterizaiton of murine cell surface components. I. Purification of milligram quantities of Thy-1.1.
    J Exp Med. 1977 Oct 1;146(4):986-1000 PMID: 302318
  14. Expression of two differentiation antigens on normal and cultured human T cells.
    J Immunol. 1978 Aug;121(2):513-9 PMID: 355545
  15. Purification and characterization of mouse brain Thy-1 glycoprotein antigens.
    J Immunol. 1978 Nov;121(5):1718-25 PMID: 81852
  16. Purification and characterization of mouse brain Thy-1.2 differentiation alloantigen.
    Brain Res. 1978 Dec 22;159(1):161-71 PMID: 83175
  17. Harvesting the products of cell growth.
    Methods Enzymol. 1979;58:221-9 PMID: 423763
  18. Identification and unusual tissue distribution of the canine and human homologues of Thy-1 (theta).
    J Exp Med. 1979 Mar 1;149(3):576-91 PMID: 311810
  19. Heterogeneity of cell surface xenoantigens on human T lymphocytes.
    J Reticuloendothel Soc. 1979 Jun;25(6):635-51 PMID: 385868
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES.
    J Exp Med. 1964 Sep 1;120:413-33 PMID: 14207060
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.151.2.400
Published
1980-02-01
Pages
400-6
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185777
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.151.2.400 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2