Chemical studies on the silver staining of nucleoli.

Experientia ·Vol. 36 ·No. 2 ·1980-02-15 ·Pages 249-51

Kling H, Lepore L, Krone W, Olert J, Sawatzki G

Abstract

The uptake of Ag-ions by isolated nucleoli of rat liver cells was studied. Nucleolar proteins separated by electrophoresis were examined for selective silver-staining. A mechanism for preferential Ag-staining of the nucleoli is discussed.

MeSH Terms

Animals Biological Transport Cell Nucleolus/analysis,metabolism Histocytochemistry Liver/cytology,metabolism Nucleoproteins/analysis Rats Silver/metabolism Staining and Labeling

Chemicals

Nucleoproteins Silver

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kling H

Lepore L

Krone W

Olert J

Sawatzki G

References (18)

18 references, click to expand

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On the complexing of deoxyribonucleic acid by silver (I).
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Studies on metal ions-DNA interactions: specific behaviour of reiterative DNA sequences.
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Surface ultrastructure and cytochemistry of isolated nucleoli from Novikoff hepatoma ascites cells.
Exp Cell Res. 1975 Oct 15;95(2):333-46 PMID: 1193156
Differential staining of the satellite regions of human acrocentric chromosomes.
Experientia. 1975 Feb 15;31(2):260-2 PMID: 46207
Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and nucleotides.
Chem Rev. 1971 Oct;71(5):439-81 PMID: 5126179
Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.
Chromosoma. 1975 Nov 20;53(1):37-50 PMID: 53131
The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.
Biochim Biophys Acta. 1962 May 14;55:571-83 PMID: 13893456
Demonstration of a non-RNA nucleolar fraction by silver staining.
Exp Cell Res. 1962 Mar;26:428-31 PMID: 13883625
Ultrastructural studies on RNA and DNA components of isolated nucleoli of Walker 256 carcinosarcoma.
Exp Cell Res. 1966 Jan;41(1):81-98 PMID: 4952036
The nature of the Ag-staining of nucleolus organizer regions. Electron- and light-microscopic studies on human cells in interphase, mitosis, and meiosis.
Cytogenet Cell Genet. 1978;20(1-6):24-39 PMID: 77208
Fractionation of nucleolar proteins by two-dimensional gel electrphoresis.
Can J Biochem. 1976 Jan;54(1):9-14 PMID: 3272
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Isolation of nucleoli from rat liver in the presence of magnesium ions.
Exp Cell Res. 1972 Mar;71(1):65-74 PMID: 4112658
The reaction of nucleoli with ammoniacal silver nitrate in darkness; additional data.
J Histochem Cytochem. 1955 May;3(3):196-202 PMID: 14381607
Cytological and histochemical studies on the mechanism of the selective silver staining of nucleolus organizer regions (NORs).
Histochemistry. 1979 Feb 26;60(1):91-9 PMID: 85618
The silver-reducing property of the nucleolus and the formation of prenucleolar material during mitosis.
Exp Cell Res. 1959 Jun;17(3):560-4 PMID: 13672223
Substances interfering with spectrophotometric estimation of nucleic acids and their elimination by the two-wavelength method.
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Article Info

Journal

Experientia

Abbr.

Experientia

ISSN

0014-4754

DOI

10.1007/BF01953762

Published

1980-02-15

Pages

249-51

Language

English

Region

Switzerland

NLM ID

0376547

Subset

External Links

PubMed source DOI: 10.1007/BF01953762

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Chemical studies on the silver staining of nucleoli.

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