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PMID: 6154931 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structure of the alpha-fetoprotein gene in the mouse.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 3 ·1980-03-00 ·Pages 1351-5

Gorin MB, Tilghman SM

Abstract

The mouse alpha-fetoprotein mRNA is the product of a single-copy gene whose mRNA coding sequences are represented discontinuously in the genome. Several EcoRI genomic fragments which contain portions of the alpha-fetoprotein gene have been cloned using the EK2 vector lambda gt WES . lambda B. In addition, a mouse genomic library has been screened to obtain a 15.75-kilobase segment of DNA that includes more than 85% of the alpha-fetoprotein coding sequence. Analyses by restriction endonuclease mapping and electron microscopy showed that the mRNA sequence is interrupted by at least 11 intervening sequences which occupy 90% of the cloned DNA.

MeSH Terms
Animals Base Sequence Cloning, Molecular/methods Codon DNA/genetics DNA Restriction Enzymes DNA, Recombinant Genes Mice/genetics Nucleic Acid Hybridization RNA, Messenger/genetics alpha-Fetoproteins/genetics
Chemicals
Codon DNA, Recombinant RNA, Messenger alpha-Fetoproteins DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gorin M B
Tilghman S M
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.3.1351
Published
1980-03-00
Pages
1351-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348492
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.3.1351 PMC Full Text View journal details
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