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PMID: 6156454 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Insulin-treated 3T3-L1 adipocytes and cell-free extracts derived from them incorporate 32P into ribosomal protein S6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2641-5

Smith CJ, Rubin CS, Rosen OM

Abstract

Differentiated 3T3-L1 preadipocytes preincubated for 60 min with 32Pi incorporate 32P into ribosomal protein S6 within 5 min after exposure to 0.1-1.0 nM insulin. Undifferentiated 3T3-L1 cells, which possess only 3-5% of the high-affinity cell surface insulin receptors present on the differentiated cells, are less sensitive to this stimulation. Under the same conditions used for insulin, epidermal growth factor, antibody to the insulin receptor, and a combination of isoproterenol and 1-methyl-3-isobutylxanthine also promote 32P incorporation into S6 in the differentiated cells although less effectively than insulin. Cell-free extracts derived from cells treated for 5-10 min with either physiological concentrations of insulin or epidermal growth factor (0.1 microgram/ml) reflect intact cells and catalyze the incorporation of 32P from exogenous [gamma-32P]ATP into ribosomal protein S6.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Adenosine Triphosphate/metabolism Adipose Tissue/drug effects,metabolism Animals Cell-Free System Cells, Cultured Epidermal Growth Factor/pharmacology Insulin/pharmacology Isoproterenol/pharmacology Mice Phosphorylation Protein Kinases/metabolism Ribosomal Proteins/metabolism
Chemicals
Insulin Ribosomal Proteins Epidermal Growth Factor Adenosine Triphosphate Protein Kinases Isoproterenol 1-Methyl-3-isobutylxanthine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith C J
Rubin C S
Rosen O M
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2641
Published
1980-05-00
Pages
2641-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349458
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.2641 PMC Full Text View journal details
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